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Merck

MAK064

Kit per saggio del lattato

sufficient for 100 colorimetric or fluorometric tests

Sinonimo/i:

Kit per saggio dell’acido lattico

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Informazioni su questo articolo

NACRES:
NA.84
UNSPSC Code:
12161503

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Nome del prodotto

Kit per saggio del lattato, sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

species reactivity

mouse, human

storage condition

dry at room temperature

technique(s)

activity assay: suitable

input

blood
cell

accession no.

P00338
NC_000011.10
NC_000073.7

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

Gene Information

human ... LDHA(3939)
mouse ... Ldha(16828)

Application

Adatto al rilevamento del L(+)-lattato nel terreno di coltura, nel terreno di fermentazione, nel sangue e nelle cellule.
Il kit del saggio del lattato è stato usato per determinare la concentrazione di lattato nei campioni.[1][2]

Biochem/physiol Actions

In questo saggio, la concentrazione del lattato si determina con dosaggio enzimatico, che fornisce un prodotto colorimetrico (570 nm)/fluorimetrico (λex = 535 nm/λem = 587 nm), proporzionale al lattato presente. I livelli tipici di sensibilità per questo kit sono compresi tra 0,001 e 10 mM.

General description

Il L(+)-lattato è un composto metabolico che si forma negli animali per l′azione dell′enzima lattato deidrogenasi. Il lattato viene prodotto nelle cellule che proliferano e in condizioni anaerobiche come l′esercizio fisico. Concentrazioni elevate di lattato al di fuori della norma sono state messe in relazione a condizioni patologiche quali cancro, diabete e acidosi lattica. Il L(+)-lattato è il principale stereoisomero del lattato che si forma nel metabolismo intermedio umano ed è presente nel sangue a livelli di circa 1-2 mmol/L. Questo kit fornisce un comodo metodo di rilevamento del L(+)-lattato nei campioni biologici. Non è necessario pretrattare né purificare il campioni.

Comparable product

N° Catalogo
Descrizione
Determinazione del prezzo

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Classe di stoccaggio

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
8J15K06070
8A17K06070
8B23K06070
8L03K06070
8F30K06070

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Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
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International journal of oncology, 51(2), 498-506 (2017-07-18)
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo
Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension.
Altara R, et al.
Frontiers in Pharmacology, 7, 436-436 (2016)
Juping Zhao et al.
Molecular medicine reports, 16(6), 8335-8344 (2017-10-07)
Previous studies have indicated that high expression of lactate dehydrogenase A (LDHA) exists in many human cancers. Recently, several reports showed that silencing or inhibition of LDHA could suppress metastasis of human cancer including renal cell carcinoma (RCC). However, the
Khalid N M Abdelazeem et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6), 2374-2382 (2017-05-04)
Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by
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Domande

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the formula for the MAK064D Lactate standard?

    1 risposta

    1. The standard provided in the MAK064-1KT Lactate Assay Kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. It contains no other components or cofactors.

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  4. Since the L-(+)-Lactate standard is not a pure solution of sodium L-(+)-Lactate in water or buffer, which are the cofactors present in the standard?

    1 risposta

    1. The standard provided in this kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. There are no other components or cofactors present in the standard.

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  5. What is the concentration range of lactate for this? The description says typical sensitivity is 0.001 - 10 mM, but in the product info the standard curve for colorimetric detection appears to go to a max. concentration of 0.0002µM (10nmol in 50µL sample)

    1 risposta

    1. The lower limit of detection is calculated by dividing the lowest concentration standard by the maximum sample volume. The higher limit of detection is determined by dividing the highest concentration standard by the minimum sample volume.

      For the higher limit of detection, take the 10 nmol standard and divide by 1 uL to get 10 mM. For the lower limit of detection, take 0.02 nmol and divide by 50 uL to get 0.0004 mM. We have reached out to our vendor to discuss the range calculation further, as it seems there is some conflicting information.

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  6. Can this kit be used on conditioned media containing phenol red?

    1 risposta

    1. If the sample being measured is the cell culture supernatant, the required volume is 20 uL. This is diluted with a total volume of 100 uL of the various kit reagents. The presence of phenol red may impact the results. It would be up to the end user to determine suitability.

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  7. On which enzymatic reaction is this kit based? Does pyruvate in the sample interfere with the assay?

    1 risposta

    1. This kit utilizes lactate oxidase to measure lactate. In sufficient amounts pyrivate may impact the results. This can be corrected by subtracting any background from the blank value. See page 3 in the kit protocol provided in the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/411/763/mak064bul.pdf

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  8. What is the Department of Transportation shipping information for this product?

    1 risposta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  9. Can I make up my own Lactate standard to use with Product MAK064, Lactate Assay kit?

    1 risposta

    1. Unfortunately, no. If you run the 100 mM L-(+)-Lactate standard (MAK064D) that comes with the kit, you should get good results. However, if you just use, for example, a solution of sodium L-(+)-lactate dissolved in water or buffer, you probably won't get good results. There are cofactors present in biological samples and in the L-(+)-Lactate standard that comes with the kit (which is not a pure solution of sodium L-(+)-Lactate in water or buffer). If such a "homemade" standard solution were to be spiked with a biological sample or the kit standard, the calculations would be more complicated, but the assay should work.

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