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Merck

N3910

Neutralization Solution B

Sinonimo/i:

DNA neutralization solution, neutralization buffer DNA extraction

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Informazioni su questo articolo

Codice UNSPSC:
12161703
NACRES:
NA.25

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Grado

Molecular Biology

Stato

liquid

tecniche

DNA amplification: suitable
DNA extraction: suitable

Temperatura di conservazione

−20°C

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Questo articolo
N9784SRE0087D5688
Neutralization Solution B

Sigma-Aldrich

N3910

Neutralization Solution B

Neutralization Solution for Blood sufficient for 100 reactions, Molecular Biology, sufficient for 1000 reactions

Sigma-Aldrich

N9784

Neutralization Solution for Blood

Sigma-Aldrich

Sigma-Aldrich

SRE0087

Neutralization Solution for Blood

Dilution Solution Extract-N-Amp Plant Dilution Solution

Sigma-Aldrich

D5688

Dilution Solution

technique(s)

DNA amplification: suitable, DNA extraction: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

Quality Level

200

Quality Level

200

Quality Level

500

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C

Descrizione generale

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Applicazioni

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp™ extraction procedure.[1] It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp™ PCR Reaction Mix.[2]

Note legali

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pittogrammi

Exclamation markEnvironment
GHS07,GHS09

Avvertenze

Warning

Indicazioni di pericolo

H315,H317,H319,H411

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000470472
0000470472
0000318607
0000381622
0000381620

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American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
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Protocolli

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

Domande

Recensioni

Nessuna valutazione

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