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Merck

N8016

2-Nitrophenyl β-D-glucopyranoside

≥99% (TLC), powder

Sinonimo/i:

2-Nitrophenyl-beta-D-glucopyranoside, o-NPG

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250 MG

229,00 €

1 G

702,00 €

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Informazioni su questo articolo

Formula empirica (notazione di Hill):
C12H15NO8
Numero CAS:
2816-24-2
Peso molecolare:
301.25
NACRES:
NA.28
PubChem Substance ID:
24897848
UNSPSC Code:
12352204
EC Number:
220-568-5
MDL number:
MFCD00065048
Beilstein/REAXYS Number:
92206

Documenti fondamentali

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Nome del prodotto

2-Nitrophenyl β-D-glucopyranoside, powder

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1

InChI key

KUWPCJHYPSUOFW-RMPHRYRLSA-N

SMILES string

OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

assay

≥99% (TLC)

form

powder

solubility

water: 50 mg/mL

storage temp.

−20°C

Quality Level

300

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Questo articolo
N7006N9376N1377
2-Nitrophenyl β-D-glucopyranoside powder

Sigma-Aldrich

N8016

2-Nitrophenyl β-D-glucopyranoside

4-Nitrophenyl β-D-glucopyranoside ≥98% (TLC)

Sigma-Aldrich

N7006

4-Nitrophenyl β-D-glucopyranoside

4-Nitrophenyl N-acetyl-β-D-glucosaminide ≥99% (TLC)

Sigma-Aldrich

N9376

4-Nitrophenyl N-acetyl-β-D-glucosaminide

4-Nitrophenyl α-D-glucopyranoside ≥99%

Sigma-Aldrich

N1377

4-Nitrophenyl α-D-glucopyranoside

assay

≥99% (TLC)

assay

≥98% (TLC)

assay

≥99% (TLC)

assay

≥99%

solubility

water: 50 mg/mL

solubility

water: 50 mg/mL, clear to very slightly hazy

solubility

water: 5 mg/mL, clear, colorless to very faintly yellow

solubility

methanol: 20 mg/mL, clear to very slightly hazy, colorless to greenish-yellow

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

form

powder

form

powder

form

powder

form

powder or crystals

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

2-Nitrophenyl β-D-glucopyranoside has been used as a substrate for glucosidase in protein assay[1] and in a colorimetric assay to determine protein stability.[2]

Biochem/physiol Actions

2-Nitrophenyl β-D-glucopyranoside serves as an artificial substrate for glucosidase.[1]

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000483140
0000483140
0000439943
0000439943
SLCM8666

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Neil J Nosworthy et al.
Enzyme and microbial technology, 54, 20-24 (2013-11-26)
We have achieved plasma immersion ion implantation (PIII) treatment of beads and powders using a specially designed plasma treatment system. This simple one-step production of functionalized beads provides an attractive alternative to current commercial functional beads, for which proteins must
J H Lebbink et al.
Biochemistry, 39(13), 3656-3665 (2000-03-29)
The beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus (CelB) is the most thermostable and thermoactive family 1 glycosylhydrolase described to date. To obtain more insight in the molecular determinants of adaptations to high temperatures and study the possibility of optimizing
Antonio Trincone et al.
Chembiochem : a European journal of chemical biology, 6(8), 1431-1437 (2005-07-13)
Transglycosylation reactions (autocondensation of the substrate or transfer of the glycon donor moiety to different acceptors) with the hyperthermophilic glycosynthase from Sulfolobus solfataricus acting in dilute sodium formate buffer at pH 4.0 are reported; the use of 4-nitrophenyl beta-glucopyranoside as
M Moracci et al.
Biochemistry, 37(49), 17262-17270 (1998-12-23)
The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst.
L M Hernandez et al.
FEBS letters, 161(2), 190-194 (1983-09-19)
Accumulation and secretion of beta-glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18-1). When incubated at the restrictive temperature no accumulation of active glucanases was observed.
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Domande

  1. hello, is this also a chromogenic substrate?

    1 risposta

    1. Yes, this compound is considered to be a chromogenic beta-glucosidase substrate. Application testing is not performed on this product. Please see the links below to review publications that may be helpful.

      Characterization of a heat resistant ß-glucosidase as a new reporter in cells and mice
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909947/

      Ion implantation treatment of beads for covalent binding of molecules: Application to bioethanol production using thermophilic beta-glucosidase
      https://www.sciencedirect.com/science/article/pii/S0141022913002019?via%3Dihub

      Utile?

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