Percoll is produced by Cytiva, formerly known as GE Life Sciences. Details regarding the proprietary manufacturing process are not publicly available. However, it can be mentioned that the Percoll particles range in size from 15 to 30 nanometers. While the density of Percoll solution is measured, the specific information regarding the amount of coating on the silica particles is not provided.
P1644
Percoll®
pH 8.5-9.5 (20 °C)
Sinonimo/i:
Mezzo a gradiente di densità Percoll
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25 ML
69,80 €
100 ML
198,00 €
500 ML
606,00 €
1 L
1.060,00 €
Informazioni su questo articolo
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Permettici di aiutartiSterilità
aseptically filled
Livello qualitativo
Stato
colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
tecniche
cell culture | mammalian: suitable
Colore
clear colorless to faint yellow
pH
8.5-9.5 (20 °C)
applicazioni
hematology
histology
Temperatura di conservazione
2-8°C
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Questo articolo | P4937 | P7828 | A8577 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | plant: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable |
| form colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) ) | form colloidal | form colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) | form solution |
| sterility aseptically filled | sterility aseptically filled | sterility aseptically filled | sterility aseptically filled |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color clear colorless to faint yellow | color - | color - | color - |
Descrizione generale
Percoll® è un classico terreno per la centrifugazione a gradiente di densità di cellule, virus e particelle subcellulari. Percoll è costituito da particelle di silice colloidale di 15-30 nm di diametro (23% p/p in acqua) rivestite con polivinilpirrolidone (PVP). Il rivestimento in PVP rende il prodotto totalmente non tossico e ideale per l′uso con materiali biologici.
Applicazioni
Percoll è costituito da particelle di silice colloidale di 15-30 nm di diametro (23% p/p in acqua) rivestite con polivinilpirrolidone (PVP). È usato nella separazione cellulare e nell′isolamento degli organuli.
Percoll è costituito da particelle di silice colloidale di 15-30 nm di diametro (23% p/p in acqua) rivestite con polivinilpirrolidone (PVP). È usato per definire gradienti di densità non tossici a bassa viscosità, bassa osmolarità utilizzabili per isolare cellule, organuli e virus. È stato usato per separare le vescicole di membrana interne ed esterne da E. coli. Percoll è isosmotico lungo tutto il gradiente, non è tossico e si rimuove con facilità dai materiali purificati.
Percoll® è stato usato nella preparazione dello sperma per studiare le richieste di energia degli embrioni bovini.[1]
Note legali
Percoll is a registered trademark of Cytiva
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves
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Rat liver mitochondria were isolated by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation, as assessed by marker enzyme analysis, latency of cytochrome-c oxidase, respiratory control index and electron microscopy. Two different
Articoli
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Protocolli
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Contenuto correlato
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What is the details about the manufacturing process of Percoll, specifically regarding the amount of PVP used during bead soaking or the approximate PVP concentration within Percoll?
1 risposta-
Utile?
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What is procedure for sperm preparation for ivf with percoll gradient
1 risposta-
Please note that this product is intended for research use only. This material is not tested for the isolation of sperm samples. The following reference may be helpful:
Chen. Y. et al., Int. J. Fertil., 37, 315-319 (1992).Please see the link below to review the product datasheet for additional information.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdfUtile?
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CAN I FREEZE PERCOLL (P1644)? I SEE IN OTHER REF THAT I CAN, BUT NOT IN THIS REF
1 risposta-
This material should not be stored frozen for long periods of time. As per the product information sheet, if stored at –20 °C, gradients form upon thawing, necessitating mixing the contents of the bottle before use. Please see the link below to review the product datasheet for further information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfUtile?
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Percoll (P1644) 을 gradient 만드는데 사용했는데요, P4937도 같은 용도로 사용 가능한가요?
1 risposta-
Yes, product P4937 can be used for the same use as product P1644.
Utile?
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I often find crystals. Can I filter the solution before use? What filter size could I use without affecting the functionality of the product?
1 risposta-
Particles may be removed by filtration or low speed centrifugation. More information about the particles filtration can be found in the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfUtile?
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What is the difference between Percoll® Products P4937 and P1644?
1 risposta-
Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.
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Can you help me calculate gradients for Percoll®, Product P1644?
1 risposta-
The product information sheet (under Documents, above) has an equation for calculating density of a given solution.
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Can Percoll®, Product P1644, be autoclaved?
1 risposta-
Percoll can be autoclaved, but this must be done without any salts or sucrose present.
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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When using Product P1644, Percoll®, what density should I use for my particular system?
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The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.
Utile?
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