The solubility of this product in DMSO has not been tested. A 200 mM stock solution can be prepared using ethanol, methanol, and 2-propanol. It is known to have 100 mM solubility in DMSO. The product is insoluble in aqueous solutions. Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C. Storing in a desiccator may be useful.
P7626
Fenil metansolfonile fluoruro
powder, ≥98.5% (GC)
Sinonimo/i:
αP-toluensolfonile fluoruro, Benzilsolfonile fluoruro, Fenil metilsolfonile fluoruro, PMSF
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250 MG
29,70 €
1 G
72,30 €
5 G
152,00 €
25 G
417,00 €
100 G
1.350,00 €
29,70 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C7H7FO2S
Numero CAS:
Peso molecolare:
174.19
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352202
EC Number:
206-350-2
MDL number:
Beilstein/REAXYS Number:
2088311
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Permettici di aiutartiNome del prodotto
Fenil metansolfonile fluoruro, ≥98.5% (GC)
InChI key
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
SMILES string
FS(=O)(=O)Cc1ccccc1
biological source
synthetic
assay
≥98.5% (GC)
form
powder
mp
91-94 °C
solubility
dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.)
H2O: unstable
Quality Level
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
1 of 4
Questo articolo | PMSF-RO | 78830 | 52332 |
|---|---|---|---|
| biological source synthetic | biological source - | biological source synthetic | biological source - |
| assay ≥98.5% (GC) | assay 98% (TLC) | assay ≥99.0% (T) | assay ≥99% (GC) |
| Quality Level 300 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form powder | form solid | form powder or crystals | form crystalline solid |
| solubility dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.), H2O: unstable | solubility >10 mg/mL | solubility anhydrous isopropanol: soluble 35 mg/mL, clear to faintly hazy, colorless to faintly yellow (Heating required) | solubility ethanol: soluble, isopropanol: soluble, methanol: soluble |
| mp 91-94 °C | mp 92-94 °C | mp 92-95 °C | mp - |
Application
Il fenil metansolfonile fluoruro è stato usato nelle seguenti applicazioni:
Spesso il PMSF è usato nel tampone di lisi per contribuire alla conservazione delle proteine di interesse durante l′isolamento delle proteine e la preparazione del campione, inibendo le proteasi che altrimenti degraderebbero le proteine dopo la lisi delle cellule o dei tessuti. Nei terreni acquosi il PMSF è instabile. Le soluzioni madre di PMSF vengono quindi preparate di solito in solventi organici anidri (per es. etanolo o isopropanolo anidro al 100%) prima dell′uso in terreni acquosi.
Le caratteristiche generali e i vantaggi osservati del PMSF includono:
Le caratteristiche generali e i vantaggi osservati del PMSF includono:
- Inibisce le serin proteasi quali tripsina e chimotripsina
- Inibisce anche le cisteina proteasi (reversibile con tioli ridotti) e l′acetilcolinesterasi di mammifero
- Non altrettanto efficace o tossico del DPF
- Concentrazione efficace 0,1-1 mM
- Emivita = 1 ora a pH 7,5
Biochem/physiol Actions
La somministrazione di PMSF produce analgesia non correlata all′effetto anticolinesterasico, e prolunga l′effetto analgesico della β-endorfina somministrata a livello centrale.[5]
General description
Il fenil metansolfonile fluoruro (PMSF) è un inibitore della serin proteasi ampiamente usato, efficace nei confronti di enzimi quali chimotripsina, trombina e tripsina. Il PMSF agisce da inibitore attraverso la solfonazione dei residui idrossilici dei residui di serina presso i siti reattivi della serin proteasi.
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Danger
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Hazard Classifications
Acute Tox. 3 Oral - Skin Corr. 1B
Classe di stoccaggio
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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C Pinsky et al.
Life sciences, 31(12-13), 1193-1196 (1982-09-20)
Intraperitoneal (IP) injection of the serine proteinase inhibitor phenylmethylsulfonyl fluoride (PMSF) produced dose-dependent analgesia in Sprague-Dawley rats. AD50 was 2.9 +/- 1.4 (S.E.) mg kg-1, the analgesia was antagonized by naloxone but unaffected by atropine. PMSF significantly enhanced the analgesic
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Contenuto correlato
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Can this PMSF powder be dissolved in DMSO, and if yes, then to what concentration where and how to store the reconstituted vial?
1 risposta-
Utile?
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At what temperature should the stock Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?
1 risposta-
A 200 mM PMSF solution in dry solvent has been reported to remain active for at least 9 months at
2-8 °C. 100 mM PMSF solutions in isopropanol may be stored at –20 °C for several months. For more information please view the Product Data Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/427/p7626pis-ms.pdfUtile?
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How should stock solutions of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?
1 risposta-
A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C.
Utile?
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What is the effective amount of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), to be used?
1 risposta-
The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?
1 risposta-
Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water.
Utile?
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What does Product P7626, Phenylmethanesulfonyl fluoride (PMSF), inhibit?
1 risposta-
PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.
Utile?
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