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Merck

S1452

MISSION® siRNA Transfection Reagent

Sinonimo/i:

MISSION® siRNA Transfection Reagent

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100 μL

74,90 €

1 ML

421,00 €

74,90 €


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Informazioni su questo articolo

NACRES:
NA.51
UNSPSC Code:
41106502

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product line

MISSION®

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

Quality Level

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product line

MISSION®

product line

-

product line

MISSION®

product line

MISSION®

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

−70°C

form

aqueous solution

form

liquid

form

-

form

-

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100, 200

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Classe di stoccaggio

3 - Flammable liquids

wgk

WGK 3

flash_point_f

120.2 °F

flash_point_c

49 °C


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This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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