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Merck

S5442

SyntheChol® NS0 Supplement

500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture

SyntheChol® NS0 Supplement

Sinonimo/i:

Cholesterol solution

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

Scegli un formato

2 ML

83,30 €

10 ML

280,00 €

83,30 €


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Informazioni su questo articolo

Formula empirica (notazione di Hill):
C27H46O
Numero CAS:
Peso molecolare:
386.65
UNSPSC Code:
12352211
eCl@ss:
39023139
PubChem Substance ID:
NACRES:
NA.75
MDL number:

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Nome del prodotto

SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture

SMILES string

[H][C@@]12CC=C3C[C@@H](O)CC[C@]3(C)[C@@]1([H])CC[C@]4(C)[C@H](CC[C@@]24[H])[C@H](C)CCCC(C)C

InChI key

HVYWMOMLDIMFJA-DPAQBDIFSA-N

InChI

1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1

biological source

non-animal source

sterility

sterile-filtered

product line

BioXtra

form

aqueous solution

concentration

500 ×

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Quality Level

Gene Information

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Questo articolo
1376721.04672228111
Colesterolo For Cell Culture, EMPROVE® EXPERT

SAFC

137672

Colesterolo

Colesterolo EMPROVE® EXPERT, Ph. Eur., BP, JP, NF

SAFC

1.04672

Colesterolo

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

non-animal source

biological source

synthetic

biological source

synthetic

biological source

-

form

aqueous solution

form

-

form

powder

form

powder

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

10-30°C

concentration

500 ×

concentration

-

concentration

-

concentration

-

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

100

Application

SyntheChol® NS0 Supplement is used as media supplement for NS0 mouse myeloma cell growth and the growth of other cholesterol-dependent cell lines.[1][2][3][4][5]

Features and Benefits

Synthetic, non-animal cholesterol solution that is designed as a supplement to our hybridoma media formulations to promote excellent cell growth and protein productivity for NS0 mouse myeloma and other cholesterol-dependent cell lines.

Legal Information

SyntheChol is a registered trademark of Merck KGaA, Darmstadt, Germany

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N° Catalogo
Descrizione
Determinazione del prezzo

Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articoli

Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.

Contenuto correlato

Domande

1–4 di 4 domande  
  1. What is the concentration of product S5442?

    1 risposta
    1. Unfortunately, the formulation of Product S5442 is considered proprietary.

      Utile?

  2. Is this formulation stable at room temperature? If so, how long is it stable?

    1 risposta
    1. This product ships at ambient temperature and is stable for relatively short periods (up to two weeks for transit time) at room temperature. However, refrigerated temperatures are recommended for long-term storage.
      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Utile?

  3. Could you please clarify the concentration of cholesterol in this product both at the original 500X solution and at the final 1X?

    1 risposta
    1. The concentration of synthetic cholesterol in this product is 0.25% or 2.5 mg/mL as supplied. The product is considered a 500X preparation meaning that 2 mL of this solution will supplement 1 L of culture medium. The 1X concentration, when diluted in 1L of medium, would be 5 ug/mL. Some cells may require a higher concentrations of cholesterol. The suggested usage range for this product is 2 - 6 mL per liter of medium. See the link below to review the product datasheet.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Utile?

  4. Hi, What is the concentration of Synthechol in this product in mg/ml or mol/l Thank you

    1 risposta
    1. Unfortunately, the formulation of item S5442 is proprietary. The concentration of the synthetic cholesterol is 0.25% (w/v) as supplied.

      Utile?

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