V8012

Anti-V5 antibody, Mouse monoclonal

Name: Anti-V5 antibody, Mouse monoclonal
Brand: SIGMA
Price: 401 EUR

clone V5-10, purified from hybridoma cell culture

Sinonimo/i:

V5 Tag Monoclonal Antibody, V5 antibody

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50 μG

401,00 €

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Informazioni su questo articolo

NACRES:

NA.56

UNSPSC Code:

12352203

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Nome del prodotto

Anti-V5 antibody, Mouse monoclonal, clone V5-10, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

V5-10, monoclonal

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL
western blot: 0.5-1 μg/mL

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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Questo articolo	V4014	V2260	C0992
Sigma-Aldrich V8012 Anti-V5 antibody, Mouse monoclonal	Sigma-Aldrich V4014 Anti-V5-Cy3^™ antibody, Mouse monoclonal	Sigma-Aldrich V2260 Monoclonal Anti-V5-Peroxidase antibody produced in mouse	Sigma-Aldrich C0992 Monoclonal Anti-Cy3/Cy5
antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
conjugate unconjugated	conjugate CY3 conjugate	conjugate peroxidase conjugate	conjugate unconjugated
clone V5-10, monoclonal	clone V5-10, monoclonal	clone V5-10, monoclonal	clone CY-96, monoclonal
shipped in dry ice	shipped in wet ice	shipped in -	shipped in dry ice
technique(s) immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL	technique(s) immunocytochemistry: 2.0-4.0 μg/mL	technique(s) western blot: 1:4,000-1:8,000	technique(s) direct ELISA: suitable, dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide conjugated to KLH.

The mouse monoclonal Anti-V5 antibody reacts specifically with the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

The mouse mooclonal Anti-V5 antibody reacts specifically with the V5-taged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number

0000463835

0000488170

0000381742

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Contenuto correlato

Data Sheet - V8012

Instructions