Autoclaving is not recommended with glucose/dextrose according to the product information sheet for this product. However, autoclaving can be performed without the sugar, and filter sterilized glucose/dextrose can be added afterward.
Y0626
Yeast Nitrogen Base Without Amino Acids
Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements
Sinonimo/i:
ynb without amino acids
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250 G
253,00 €
1 KG
759,00 €
253,00 €
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Informazioni su questo articolo
NACRES:
NA.85
UNSPSC Code:
41106212
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Permettici di aiutartiNome del prodotto
Yeast Nitrogen Base Without Amino Acids, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements
grade
Molecular Biology
sterility
non-sterile
form
powder
technique(s)
microbe id | utilization test: suitable
microbiological culture: suitable
pH
5.2-5.6(0.67% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
yeasts
Quality Level
1 of 4
Questo articolo | Y1250 | Y1251 | 51483 |
|---|---|---|---|
| suitability nonselective for Candida spp., nonselective for Saccharomyces spp., yeasts, nonselective for Pichia spp., nonselective for Zygosaccharomyces spp. | suitability nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp. | suitability Candida spp., Pichia spp., Saccharomyces spp., Zygosaccharomyces spp., molds, yeasts | suitability fungi, selective by low pH for Candida spp., selective by low pH for Pichia spp., selective by low pH for Saccharomyces spp., selective by low pH for Zygosaccharomyces spp., selective by low pH for molds (General Media), selective by low pH for yeasts (General Media) |
| technique(s) microbe id | utilization test: suitable, microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbe id | susceptibility testing: suitable, microbe id | utilization test: suitable, microbiological culture: suitable |
| form powder | form powder | form powder | form powder |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) microbiology | application(s) clinical testing | application(s) agriculture |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.
Yeast Nitrogen Base Without Amino Acids has been used:
- in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing[1] and characterization[2]
- as a component of selective liquid media for uracil auxothrophic growth in yeast[3]
- to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions[4]
General description
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.
Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.[5]
Other Notes
Component, amount (μg/L unless indicated)
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L
Preparation Note
1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.
related product
N° Catalogo
Descrizione
Determinazione del prezzo
Classe di stoccaggio
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Instructions
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Why is autoclaving this not recommended?
1 risposta-
Utile?
-
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Can this be autoclaved, or only filter sterilized?
1 risposta-
It is not recommended to autoclave Product Y0626 Yeast Nitrogen Base Without Amino Acids. If preparing an bacteriological agar, then the agar should be autoclaved separately for 15 minutes only. Please follow the instructions listed on the product detail page:
1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.Utile?
-
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