05-1087

Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2

Name: Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2
Brand: MM

clone 24.6.2, from mouse

別名:

insulin receptor substrate 1

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この商品について

UNSPSCコード:

12352203

eCl@ss:

32160702

NACRES:

NA.41

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由来生物

mouse

品質水準

100

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

24.6.2, monoclonal

交差性

bovine, pig, mouse, monkey, canine, rat, human

テクニック

immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

アイソタイプ

IgG2aκ

NCBIアクセッション番号

NP_005535.1

UniProtアクセッション番号

P35568

輸送温度

wet ice

ターゲットの翻訳後修飾

phosphorylation (pSer307 )

遺伝子情報

bovine ... Irs1(538598)
dog ... Irs1(486148)
human ... IRS1(3667)
mouse ... Irs1(16367)
pig ... Irs1(100512686)
rat ... Irs1(25467)
rhesus monkey ... Irs1(707870)

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当該品目	05-1086	05-1085	05-784R
Sigma-Aldrich 05-1087 Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2	Sigma-Aldrich 05-1086 Anti-phospho-IRS1 (Ser302 mouse/ Ser307 human) Antibody, clone 8.1.2	Sigma-Aldrich 05-1085 Anti-IRS1 Antibody, clone 4.2.2	Sigma-Aldrich 05-784R Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
clone 24.6.2, monoclonal	clone 8.1.2, monoclonal	clone 4.2.2, monoclonal	clone 58-10C-31, monoclonal
species reactivity bovine, pig, mouse, monkey, canine, rat, human	species reactivity bovine, pig, canine, monkey, human, rat, mouse	species reactivity pig, canine, human, mouse, rat, monkey, bovine	species reactivity human, rat, mouse
antibody form purified antibody	antibody form purified antibody	antibody form purified antibody	antibody form purified immunoglobulin
UniProt accession no. P35568	UniProt accession no. P35568	UniProt accession no. P35568	UniProt accession no. P35570
technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunofluorescence: suitable, western blot: suitable	technique(s) immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) immunoprecipitation (IP): suitable, western blot: suitable

詳細

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

~185 kDa

免疫原

Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1.

アプリケーション

This Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2 is validated for use in WB, IP, IC, IF for the detection of phospho-IRS1 (Ser307 mouse/ Ser312 human).

生物化学的／生理学的作用

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

This antibody recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human).

物理的形状

Format: Purified

アナリシスノート

Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 07-247

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

05-1087:

試験成績書（COA）

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MicroRNA-181b Improves Glucose Homeostasis and Insulin Sensitivity by Regulating Endothelial Function in White Adipose Tissue.

Xinghui Sun et al.

Circulation research, 118(5), 810-821 (2016-02-03)

The pathogenesis of insulin resistance involves dysregulated gene expression and function in multiple cell types, including endothelial cells (ECs). Post-transcriptional mechanisms such as microRNA-mediated regulation of gene expression could affect insulin action by modulating EC function. To determine whether microRNA-181b

Inhibition of JNK suppresses autophagy and attenuates insulin resistance in a rat model of nonalcoholic fatty liver disease.

Hua Yan et al.

Molecular medicine reports, 15(1), 180-186 (2016-12-03)

Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease, the pathological process of which is complex. Activation of the c‑Jun N‑terminal kinase (JNK) signaling pathway is associated with the mechanism underlying obesity-induced insulin resistance. Furthermore, the JNK signaling

Nicotinamide Mononucleotide Prevents Free Fatty Acid-Induced Reduction in Glucose Tolerance by Decreasing Insulin Clearance.

Ashraf Nahle et al.

International journal of molecular sciences, 22(24) (2021-12-25)

The NAD-dependent deacetylase SIRT1 improves β cell function. Accordingly, nicotinamide mononucleotide (NMN), the product of the rate-limiting step in NAD synthesis, prevents β cell dysfunction and glucose intolerance in mice fed a high-fat diet. The current study was performed to

Physical inactivity induces insulin resistance in plantaris muscle through protein tyrosine phosphatase 1B activation in mice.

Saori Kakehi et al.

Frontiers in physiology, 14, 1198390-1198390 (2023-06-30)

Inactivity causes insulin resistance in skeletal muscle and exacerbates various lifestyle-related diseases. We previously found that 24-h hindlimb cast immobilization (HCI) of the predominantly slow-twitch soleus muscle increased intramyocellular diacylglycerol (IMDG) and insulin resistance by activation of lipin1, and HCI

Inhibition of Protein Tyrosine Phosphatase 1B Improves IGF-I Receptor Signaling and Protects Against Inflammation-Induced Gliosis in the Retina.

Ana I Arroba et al.

Investigative ophthalmology & visual science, 56(13), 8031-8044 (2016-01-01)

Insulin-like growth factor-I receptor (IGF-IR) signaling mediates retinal growth and survival and its failure may contribute to aggravate diabetic retinopathy (DR). Protein tyrosine phosphatase 1B (PTP1B) negatively modulates IGF-IR signaling, but its involvement in inflammation during DR remains unknown. We

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