324393

Anti-Dopamine D₂ Receptor Rabbit pAb

lyophilized, Calbiochem^®

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: lyophilized
• 含まれません: preservative
• 交差性: rat, human
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• dilution: (ELISA ; Immunoblotting (1:800); Frozen Sections (1:5000, )
• アイソタイプ: IgG
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DRD2(1813) ; rat ... Drd2(24318)

詳細

詳細

Anti-Dopamine D₂ Receptor, rabbit polyclonal, specifically recognizes the dopamine D2 receptor in rat brain. Has minimal reactivity with the D2S receptor. It is validated for use in ELISA, WB, & IHC.

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa dopamine D₂ receptor protein.

Recognizes the dopamine D₂ receptor in rat brain. Does not cross-react with other dopamine receptors and exhibits minimal cross-reactivity with the D_2S short receptor.

免疫原

Human

a synthetic peptide (CAARRAQELEME) corresponding to amino acids 272-282 of human D₂ dopamine receptor

アプリケーション

ELISA (see comments)

Immunoblotting (1:800)

Frozen Sections (1:5000, see application references)

物理的形状

Undiluted serum.

調製ノート

Reconstitute lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

アナリシスノート

Positive Control
Rat brain

その他情報

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.

This antibody is highly specific for the immunizing peptide sequence and is suitable for detection of both the D₂S and D₂L dopamine receptors in human and rat samples. This antibody has also been reported to work for ELISA. For immunoblotting, a 1:800 dilution of the reconstituted antiserum detects two bands at ~48 and ~51 kDa in an analysis using whole rat brain homogenates. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections, a 1:5000 dilution of the reconstituted antiserum demonstrates specific staining of rat brain sections including the plasma membrane in the cortex, caudate putamen, globus pallidus, thalamic nucleus, supra optic nucleus, and the dentate gyrus. Variables associated with assay conditions will dictate the proper working dilution.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Toxicity: Standard Handling (A)

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable