616370-M

Aprotinin, Bovine Lung, Crystalline

Aprotinin, Bovine Lung, Crystalline, CAS 9087-70-1, is a competitive, reversible, heat- and acid-stable inhibitor of proteolytic and esterolytic activities.

• 別名: Aprotinin, Bovine Lung, Crystalline, Pancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor
• CAS番号: 9087-70-1
• MDL番号: MFCD00130541
• UNSPSCコード: 12352202
• NACRES: NA.25

特性

• 品質水準: 200
• 詳細: Merck USA index - 14, 757
• アッセイ: ≥95% (gel filtration chromatography)
• フォーム: crystalline solid
• 比活性: ≥5500 KIU/mg dry wt
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic)
• 不純物: ≤10 EU/mg endotoxin
• 色: white to off-white
• pI: 10.5
• 溶解性: water: 10 mg/mL
• 保管温度: 2-8°C
• SMILES記法: S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O
• InChI: 1S/C284H432N84O79S7/c1-21-144(9)222-271(439)337-174(68-46-105-309-282(300)301)239(407)340-187(120-160-77-85-164(374)86-78-160)251(419)341-185(116-156-55-29-24-30-56-156)250(418)342-188(121-161-79-87-165(375)88-80-161)252(420)346-191(123-208(291)378)246(41
• InChI Key: ZPNFWUPYTFPOJU-UHFFFAOYSA-N

詳細

詳細

A competitive and reversible inhibitor of esterase and protease activity. Forms a tight complex with and blocks the active site of target enzymes. Inhibits a number of different proteases, including chymotrypsin, coagulation factors involved in the pre-phase of blood clotting, kallikrein (K_d = 1 X 10^-7 M), plasmin (K_d = 2.3 X 10^-10 M), tissue and leukocyte proteinases, and trypsin (K_d = 5 X 10^-14 M). Does not inhibit Factor Xa or thrombin. Reported to be relatively acid and heat stable. Effectively inhibits target proteases at equimolar concentration. Useful as a serine proteinase inhibitor during purification of proteins and in studies of zymogen activation systems. Reported to suppress virus HA cleavage and limit reproduction of human and avian influenza viruses with a single arginine in the HA cleavage site.

Effectively inhibits target proteases at equimolar concentration.

Note: 1 KIU = 0.025 antiplasmin units (APU) = 0.0016 trypsin inhibitor units (using porcine trypsin) = 0.0009756 trypsin inhibitor units (using bovine trypsin).

生物化学的／生理学的作用

Kd = 1 x 10-7 M, 2.3 x 10-10 M, 5 x 10-14 M, against kallikrein, plasmin, and trypsin, respectively

Primary Target
chymotrypsin

調製ノート

Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

その他情報

One Kallikrein Inhibitory Unit (K.I.U.) is defined as the quantity of protease inhibitor that will inhibit two kallikrein units by 50% under optimal conditions. Note: 1 KIU = 0.025 antiplasmin units (APU) or 0.0031 trypsin inhibitor units.

Zhirnov, O.P., et al. 2011. Antiviral Res.92, 27.
Roberts, R.F., et al. 1998. Ann. Thorac. Surg.66, 225.
Azougagh Oualane, F., et al. 1992. Thromb. Res.68, 185.
Anderson, L.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 2979.
Kruithof, W.L., et al. 1986. Biochem. J.226, 631.
McKeehan, W.L., et al. 1986. J. Biol. Chem.261, 5378.
Fritz, H., and Wunderer, G. 1983. Arzneim. Forsch.33, 479.
Rijken, D.C., and Collen, D. 1981. J. Biol. Chem.256, 7035.
Kassell, B., et al. 1970. Methods Enzymol.19, 845.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Toxicity: Standard Handling (A)

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable