AB5970

Anti-Dishevelled-1 Antibody

Chemicon^®, from rabbit

• 別名: DSH, DVL1, DSH Homolog 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 交差性: human
• メーカー／製品名: Chemicon^®
• テクニック: western blot: suitable
• 入力: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• NCBIアクセッション番号: NM_004421.2; NM_181870.1; NM_182779.3
• UniProtアクセッション番号: O14640
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DVL1(1855)

詳細

詳細

85 kDa

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.

免疫原

Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.

アプリケーション

Research Category
幹細胞研究

Research Sub Category
造血幹細胞

神経幹細胞

Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.

Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

生物化学的／生理学的作用

Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.

物理的形状

Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

調製ノート

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

アナリシスノート

Control
SK-N-SH (neuroblastoma) whole cell lysates

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable