IP02
Protein A Agarose Suspension
Protein A Agarose Suspension designed for immunoprecipitation applications
ログインで組織・契約価格をご覧ください。
サイズを選択してください
表示を変更する
| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|
この商品について
NACRES:
NA.56
UNSPSC Code:
41116133
この製品は、Merck Milliporeウェブサイトでのみ注文できます。
form
slurry (Liquid)
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
shipped in
wet ice
storage temp.
2-8°C
General description
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG2a and IgG2b and rabbit IgG.
Protein A conjugated to agarose.
Physical form
In PBS.
Other Notes
Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
1 of 1
当該品目 | |||
|---|---|---|---|
| description Protein A Agarose Suspension designed for immunoprecipitation applications | description Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation. | description Protein G PLUS agarose suspension specifically formulated for immunoprecipitation. | description aqueous suspension |
| form slurry (Liquid) | form slurry (Liquid) | form slurry (Liquid) | form aqueous suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| contains ≤0.1% sodium azide as preservative | contains ≤0.1% sodium azide as preservative | contains ≤0.1% sodium azide as preservative | contains - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename - |
| storage condition do not freeze | storage condition do not freeze | storage condition do not freeze | storage condition - |
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable