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Merck

MAB1083

Anti-GFP Antibody, clone 3F8.2

clone 3F8.2, from mouse

別名:

Green Fluorescent protein

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この商品について

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

3F8.2, monoclonal

交差性

vertebrates

テクニック

western blot: suitable

アイソタイプ

IgG1κ

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

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当該品目
SAB4200681AB3080PMAB2510
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

clone

3F8.2, monoclonal

clone

GFP-20, monoclonal

clone

polyclonal

clone

264-449-2, monoclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

species reactivity

vertebrates

species reactivity

rat, human

species reactivity

vertebrates (most common)

species reactivity

-

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

technique(s)

western blot: suitable

technique(s)

immunoblotting: 1-2 μg/mL using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein., immunoprecipitation (IP): 5-10 μg using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein.

technique(s)

ELISA: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

詳細

28 kDa
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

免疫原

Epitope: Full Length
Full length GFP protein.

アプリケーション

Research Category
二次抗体及びコントロール抗体
Research Sub Category
エピトープタグ
Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.
Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

生物化学的/生理学的作用

Reacts with most common vertebrate proteins containing the GFP tag.
The antibody reacts with the 28 kDa GFP protein.

物理的形状

Protein G Purified
Format: Purified
Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

調製ノート

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Western Blot: GFP recombinant protein
Routinely evaluated by Western Blot on GFP recombinant protein.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MAB1083:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Ji-Long Liao et al.
Microbial biotechnology, 12(5), 920-931 (2019-06-15)
Polyketides are important secondary metabolites, many of which exhibit potent pharmacological applications. Biosynthesis of polyketides is carried out by a single polyketide synthase (PKS) or multiple PKSs in successive elongations of enzyme-bound intermediates related to fatty acid biosynthesis. The polyketide
Bernadette M DeRussy et al.
Journal of virology, 90(16), 7109-7117 (2016-05-27)
Human cytomegalovirus (HCMV) pUL93 and pUL77 are both essential for virus growth, but their functions in the virus life cycle remain mostly unresolved. Homologs of pUL93 and pUL77 in herpes simplex virus 1 (HSV-1) and pseudorabies virus (PRV) are known
Gary J Iacobucci et al.
Biophysical journal, 113(10), 2236-2248 (2017-07-18)
N-methyl-d-aspartate (NMDA) receptors are glutamate- and glycine-gated channels that flux Na+ and Ca2+ into postsynaptic neurons during synaptic transmission. The resulting intracellular Ca2+ transient is essential to physiological and pathological processes related to synaptic development, plasticity, and apoptosis. It also
Huimei Yu et al.
Nature neuroscience, 18(6), 836-843 (2015-04-29)
Contrary to the long-held belief that DNA methylation of terminally differentiated cells is permanent and essentially immutable, post-mitotic neurons exhibit extensive DNA demethylation. The cellular function of active DNA demethylation in neurons, however, remains largely unknown. Tet family proteins oxidize
Viktória K Vereczki et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(21), 4575-4595 (2021-04-11)
GABAergic neurons are key circuit elements in cortical networks. Despite growing evidence showing that inhibitory cells play a critical role in the lateral (LA) and basal (BA) amygdala functions, neither the number of GABAergic neurons nor the ratio of their

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