This product is an aqueous solution containing 35 - 55% glycerol.
76216
パパイン from Carica papaya
solution, light brown, ≥10 U/mg protein (~25 mg/ml)
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この商品について
CAS番号:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
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製品名
パパイン from Carica papaya, solution, light brown, ≥10 U/mg protein (~25 mg/ml)
biological source
papaya
form
solution
specific activity
≥10 U/mg protein (~25 mg/ml)
mol wt
Mr ~23000
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
color
light brown
storage temp.
2-8°C
Quality Level
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当該品目 | 5125-50GM-M | P5306 | 10108014001 |
|---|---|---|---|
| specific activity ≥10 U/mg protein (~25 mg/ml) | specific activity >30,000 USP units/mg | specific activity ≥8.0 units/mg protein | specific activity 30 U/mg, ~30 units/mg protein (At 25 °C with BAEE as the substrate.) |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source papaya | biological source - | biological source papaya (latex) | biological source papaya |
| form solution | form solid | form lyophilized powder | form suspension |
| mol wt Mr ~23000 | mol wt - | mol wt 23.4 kDa | mol wt 23 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. - |
Application
パパインは食肉軟化剤として使用されます。また、初代細胞培養の調製において組織を細分化するためにも使用されます。
Biochem/physiol Actions
パパインは、ペプチド結合を切断するシステインプロテアーゼであり、抗体のFc部分を切断することが知られています。
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food[4], baking, and dairy industries.[5]
Other Notes
1 unitは、pH 6.2、25°C、1分間で、1 μmolのN-ベンゾイル-L-アルギニンエチルエステル(BAEE、製品番号12880)を加水分解する酵素量に相当します。
General description
Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.[4]
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Extraction of Near-Field Fluorescence from Composite Signals to Provide High Resolution Images of Glial Cells
Robert T D, et al.
Biophysical Journal, 80(5), 2477-2482 (2001)
The effect of glycosaminoglycan enzymes and proteases on the viscosity of alpaca seminal plasma and sperm function
Kershaw CM, et al.
Animal Reproduction Science, 138(1-2), 261-267 (2013)
Papain , a plant enzyme of biological importance: a review
Mamboya E.
American Journal of Biochemistry and Biotechnology, 8(2), 99-104 (2012)
José M Laparra et al.
Journal of food science, 74(6), H168-H173 (2009-09-03)
This study aims to understand the enhancing effect of glycosaminoglycans (GAGs), such as chondroitin/dermatan structures, on Fe uptake to Caco-2 cells. High-sulfated GAGs were selectively purified from cooked haddock. An in vitro digestion/Caco-2 cell culture model was used to evaluate
Immobilization of papain: A review
Veymar G, et al.
International Journal of Biological Macromolecules, 188, 94-113 (2021)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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