This product is an aqueous solution containing 35 - 55% glycerol.
76216
パパイン from Carica papaya
solution, light brown, ≥10 U/mg protein (~25 mg/ml)
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この商品について
CAS番号:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Specific activity:
≥10 U/mg protein (~25 mg/ml)
Biological source:
papaya
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当該品目 | |||
|---|---|---|---|
| specific activity ≥10 U/mg protein (~25 mg/ml) | specific activity >30,000 USP units/mg | specific activity ≥8.0 units/mg protein | specific activity 30 U/mg, ~30 units/mg protein (At 25 °C with BAEE as the substrate.) |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source papaya | biological source - | biological source papaya (latex) | biological source papaya |
| form solution | form solid | form lyophilized powder | form suspension |
| mol wt Mr ~23000 | mol wt - | mol wt 23.4 kDa | mol wt 23 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. - |
General description
Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.
Application
パパインは食肉軟化剤として使用されます。また、初代細胞培養の調製において組織を細分化するためにも使用されます。
Papain from Carica papaya has been used:
- in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
- to study its effects on Alpaca seminal plasma viscosity and sperm function
- to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa[1]
Biochem/physiol Actions
パパインは、ペプチド結合を切断するシステインプロテアーゼであり、抗体のFc部分を切断することが知られています。
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.
Other Notes
1 unitは、pH 6.2、25°C、1分間で、1 μmolのN-ベンゾイル-L-アルギニンエチルエステル(BAEE、製品番号12880)を加水分解する酵素量に相当します。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
76216-BULK: + 76216-50MG: + 76216-1G: + 76216-250MG: + 76216-VAR:
jan
Immobilization of papain: A review
Veymar G, et al.
International Journal of Biological Macromolecules, 188, 94-113 (2021)
José M Laparra et al.
Journal of food science, 74(6), H168-H173 (2009-09-03)
This study aims to understand the enhancing effect of glycosaminoglycans (GAGs), such as chondroitin/dermatan structures, on Fe uptake to Caco-2 cells. High-sulfated GAGs were selectively purified from cooked haddock. An in vitro digestion/Caco-2 cell culture model was used to evaluate
The effect of glycosaminoglycan enzymes and proteases on the viscosity of alpaca seminal plasma and sperm function
Kershaw CM, et al.
Animal Reproduction Science, 138(1-2), 261-267 (2013)
Papain , a plant enzyme of biological importance: a review
Mamboya E.
American Journal of Biochemistry and Biotechnology, 8(2), 99-104 (2012)
Extraction of Near-Field Fluorescence from Composite Signals to Provide High Resolution Images of Glial Cells
Robert T D, et al.
Biophysical Journal, 80(5), 2477-2482 (2001)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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