It is not possible to have cells that are PI positive without the cells also being positive for Annexin V binding. Cells are PI positive because the membrane has been compromised. If this is the case, Annexin V can also enter the cell and bind to the PS on the internal cell membrane. The gating on the histogram for the FITC channel should be changed so that all cells that are PI positive are also Annexin V positive.
APOAF
アネキシンV-FITCアポトーシス検出キット
別名:
Annexin V-FITC, Apoptosis probe FITC
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usage
(20 tests)
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Quality Level
関連するカテゴリー
1 of 4
当該品目 | APOAC | S7111 | QIA39 |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) flow cytometry: suitable |
| usage (20 tests) | usage sufficient for 200 tests | usage - | usage sufficient for 50 tests |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging - |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) - |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
Application
Features and Benefits
TUNELのようなDNAベ-スの方法よりもプロセスの初期でアポト-シスを検出できます。
- 細胞を迅速にラベリング。細胞染色はわずか10分。
- 細胞の固定や処理の必要がなく、検出時間を短縮、細胞をさらに検討することも可能。
- 2次染色用の色素として、キットにはヨウ化プロピジウムが用意されており、生存細胞およびネクロ-シス細胞と、アポト-シス細胞の区別が可能。
General description
アネキシンV-FITCキットは、アポトーシス細胞に結合したアネキシンVの蛍光検出や、フローサイトメトリーによる定量測定を可能にします。 アネキシンV-FITCキットでは、膜表面のホスファチジルセリンの位置を標識するために、フルオレセインイソチオシアネート（FITC）を結合させたアネキシンVを使用します。 本キットは、細胞膜が完全に無防備となっているネクローシス細胞中のDNAを標識するためのヨウ化プロピジウム（PI）を含みます。 この組み合わせにより、初期のアポトーシス細胞（アネキシンV陽性、PI陰性）、ネクローシス細胞（アネキシンV陽性、PI陽性）、および生細胞（アネキシンV陰性、PI陰性）を区別できます。
Other Notes
使用前に全てのコンポーネントを、室温に戻してください。
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製品番号
詳細
価格
保管分類
10 - Combustible liquids
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資料
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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When using Product APOAF, Annexin V-FITC Apoptosis Detection Kit, I have cells that are Annexin V FITC negative and PI positive. What are these cells?
1 回答-
役に立ちましたか？
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Can I use Product APOAF, Annexin V-FITC Apoptosis Detection Kit, to differentiate cells that are dead due to necrosis or apoptosis?
1 回答-
When using a kinetic study (various time points), you can show the progression of the cells from viable (annexin V FITC negative, PI negative), to annexin FITC positive, PI negative (membrane flip) to annexin V FITC positive, PI positive (dead). If there are cells that are double positive when starting, it is not possible to guarantee that the cell death occurred due to apoptosis with this assay.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Can Product APOAF, Annexin V-FITC Apoptosis Detection Kit be used on fixed cells?
1 回答-
No. Product APOAF, Annexin V-FITC Apoptosis Detection Kit must be performed on live cells in order to measure Apoptosis. The assay is based on the externization of phosphatidylserine from the inner cell membrane to the outer cell membrane. If the membrane is preturbed due to fixation, non-speciifc staining of the inner cell membrane might occur
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When using the Annexin V-FITC Apoptosis Detection Kit, Product APOAF, can I use any buffer for resuspending my cells?
1 回答-
The binding buffer included in the kit needs to be used for resuspending cells. The buffer contains calcium chloride at a final (1X) concentration of 2.5 mM which is necessary for the binding of annexin V to phosphatidylserine.
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What wavelength do I use to detect Annexin V-FITC and Propidium Iodide when using Annexin V-FITC Apoptosis Detection Kit, Product APOAF?
1 回答-
Annexin V FITC will have a maximum emission of 528 nm. This can be measured in the standard FITC Channel on a flow cytometer (FL1). Propidium Iodide has a maximum emission of 620 nm. This is measured on the short red channel on a flow cytometer (FL2 or FL3).
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