The solution storage and stability of this product have not been established. However, the activated or nicked version of this material, product D4522, notes that Solutions of DNA have been stored successfully for several months at 4 °C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. Please see the link below to review additional information available in the datasheet for the D4522:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/630/619/d4522pis.pdf
D4764
デオキシリボ核酸 from calf thymus
Genomic, unsheared
別名:
DNA from calf thymus, 仔ウシ胸腺DNA, 胸腺核酸
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サイズを選択してください
0.25 UNIT
￥5,607
1 UNIT
￥16,320
5 UNITS
￥54,880
￥5,607
単価￥8,010割引30%カートの在庫状況を確認する
高品質はそのまま、さらにお手頃な価格になりました
この商品について
CAS番号:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
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当該品目 | D8899 | D4522 | D8515 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt >20 kb | mol wt ~50 kb | mol wt - | mol wt - |
Application
仔ウシ胸腺DNAは、DNAポリメラーゼアッセイの基質、非常に長い断片の増幅用、沈殿用キャリアDNAとして非常に有用です。蛍光アッセイによるDNA定量の標準物質として用いられています[1]。軟骨組織消化サンプル中のDNA含量を測定する際の標準物質として用いられています。[2][3]
仔ウシ胸腺DNAは長鎖フラグメント増幅におけるDNAポリメラ-ゼアッセイの基質として、また沈殿のキャリア-DNAとして非常に有用です。
Biochem/physiol Actions
42% GC;Tm = 87°C。
%GCはG/C塩基対の百分率を表します。Tmは、熱変性プロファイルの中点温度を表します。
%GCはG/C塩基対の百分率を表します。Tmは、熱変性プロファイルの中点温度を表します。
Features and Benefits
・ 37°Cで16時間インキュベーション後、ヌクレアーゼ活性非検出
・高品質のテンプレートDNA
・高品質のテンプレートDNA
General description
雌雄仔ウシ胸腺から単離した高品質の2本鎖鋳型DNAです。
Preparation Note
最終濃度が1 mg/mLになるまで、一晩かけて穏やかに振盪し、分子生物学グレードの水にDNAを溶解させます。
陰イオン交換クロマトグラフィーで精製し、1mM塩化ナトリウム、1mM EDTA、1mM Trizma HCl（pH7.5）の溶液で透析し、約2A260単位/mLの濃度で凍結乾燥させています。DNA1mg当たり約20A260単位。
Comparable product
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Roel J H Custers et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 28(4), 429-435 (2009-11-04)
The purposes of the present study were to explore the surgical possibilities for replacement of the medial tibial plateau by a metallic implant in a large animal model and to examine the implications for the opposing cartilage. In six goats
A Châtel et al.
Ecotoxicology (London, England), 20(8), 1727-1740 (2011-06-10)
In the present study, we analyzed the effects of two major pollutants of the environment, tributyltin (TBT) and water-accommodated fraction (WAF) of diesel oil, on MAP kinase activation, apoptosis induction and DNA damage, in the marine sponge Suberites domuncula. Our
Nathalie W D Jansen et al.
Arthritis and rheumatism, 56(1), 199-207 (2006-12-30)
Joint bleeding, or hemarthrosis, leads in time to severe joint damage. This study was carried out to test the in vitro thresholds of exposure time and concentration that lead to irreversible joint damage, to add to the discussion on the
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
Sutha Shobana et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 107, 117-132 (2013-02-19)
Equilibrium studies of Ni(II), Cu(II) and Zn(II) mixed ligand complexes involving a primary ligand 5-fluorouracil (5-FU; A) and imidazoles viz., imidazole (him), benzimidazole (bim), histamine (hist) and L-histidine (his) as co-ligands(B) were carried out pH-metrically in aqueous medium at 310±0.1K
関連コンテンツ
Datasheet
Instructions
Datasheet
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What are the storage conditions once the DNA is resuspended? Should I keep the DNA at -20ºC or -80ºC?
1 回答-
役に立ちましたか？
-
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how much is 1 unit (i.e. in ug or mg)?
1 回答-
One unit, based on the A260 value, is approximately 50 µg. The general conversion formula is 20 units per milligram. The 0.25 UN package size will contain approximately 12.5 µg, the 1 jUN package size will contain approximately 50 µg, and the 5UN package size will contain approximately 250 µg.
For additional product information, please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/289/542/d4764dat.pdf役に立ちましたか？
-
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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