F0522

モノクロナール抗CD3

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

• 別名: Monoclonal Anti-CD3
• NACRES: NA.44
• UNSPSC Code: 12352203

特性

• 製品名: モノクロナール抗CD3, clone UCHT-1, purified immunoglobulin, buffered aqueous solution
• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UCHT-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: 10 μL using 1 × 10⁶ cells
• isotype: IgG1
• UniProt accession no.: P07766
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Quality Level: 200
• Gene Information: human ... CD3E(916)

詳細

Biochem/physiol Actions

CD3複合体を認識します。クロ-ン番号UCHT-1のモノクロ-ナル抗体によって認識されるエピト-プは、CD3抗原/T細胞レセプタ-複合体のε鎖上に発現しています。3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

General description

CD3複合体(16/20/29/22/25~28 kDa)は、たがいに結合した5本のポリペプチド鎖からなり、T細胞レセプタ-と結合します。

CD3e molecule, ε (CD3E) is a polypeptide encoded by the CD3E gene in humans. It resides on chromosome 11. The CD3ε cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes and is found in Purkinje cells of the cerebellum as well as in gastric parietal cells of humans and other species. Cytoplasmic cyCD3 expression can only be seen during the early phase of NK-cell differentiation.

Immunogen

ヒト胸腺細胞、セザリ-T細胞。

Application

Monoclonal Anti-CD3-FITC antibody produced in mouse is suitable for flow cytometry (at a dilution of 10μL using 1 × 106 cells). It was also used for ADCC Assay.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable