The low value observed in the sample may be due to several factors. Since the standard curve is working well, it is likely that the lysis buffer and/or homogenization condition used is not optimized for assay reading. To verify the possible presence of inhibiting substances, one could add a known amount of ATP from a standard solution to the homogenate to see if the reading increases accordingly. It's possible that the lysis buffer used was not optimal to yield an adequate amount of ATP for detection. Currently, there is no recommended cardiac tissue preparation protocol for use with this kit. As the kit measures only soluble ATP, it is highly recommended to clarify the homogenized extract by centrifugation to obtain a supernatant solution for use with this assay.
FLAAB
アデノシン 5′-三リン酸 (ATP) アッセイミックス希釈バッファー
lyophilized powder
別名:
ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP
サイズを選択してください
| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
1 vial | カートの在庫状況を確認する | ￥17,700 |
5 vials | 在庫from市川市大町368 | ￥48,900 |
10 vials | カートの在庫状況を確認する | ￥89,700 |
この商品について
￥17,700
form
lyophilized powder
Quality Segment
technique(s)
activity assay: suitable, protein detection: suitable
storage temp.
−20°C
General description
Application
Preparation Note
1 of 1
当該品目 | |||
|---|---|---|---|
| description lyophilized powder | description lyophilized powder | description for ATP quantitation | description vial of ~1 mg ATP |
| technique(s) protein detection: suitable, activity assay: suitable | technique(s) PCR: suitable, protein detection: suitable | technique(s) - | technique(s) protein detection: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form - | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
注意喚起語
Danger
ハザードコード
Hazard Classifications
Eye Dam. 1
保管分類
11 - Combustible Solids
WGK
WGK 3
引火点 (F)
Not applicable
引火点（°C）
Not applicable
PPE（個人用保護具）
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
第一種指定化学物質
prtr
名称等を表示すべき危険物及び有害物
ishl_indicated
名称等を通知すべき危険物及び有害物
ishl_notified
FLAAB-5VL:4548173422381 + FLAAB-10VL:4548173422367 + FLAAB-1VL:4548173422374
jan
-
It seems that you are currently trying to perform the ATP assay (Sigma Cat# FLAA) on cardiac tissue. The kit appears to be working, as your standard curve is functioning, but you are obtaining very low numbers for your samples, significantly below your standard curve. Given that the heart should contain an abundance of ATP, you are concerned that your extraction protocol might be the issue. You are wondering if there is an extraction protocol available for this tissue for the mentioned kit.
1 回答-
役に立ちましたか？
-