In undiluted form or diluted to working concentration, this material may be stored at -20°C in aliquots up to the recommended retest date for the specific lot. This data is published in the Certificate of Analysis. Avoid repeated freeze/thaw cycles. The solution may be transferred to smaller vials in working volumes for long-term storage. Plastic vials are recommended as this protein tends to adhere to glass. Please see the link below to access the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/409/686/l2020pis-ms.pdf
L2020
Mouse Laminin
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 0.2 μm filtered, liquid, 1-2 mg/mL, suitable for cell culture
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
別名:
ラミニン
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製品名
ラミニン EHS肉腫基底膜由来, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture
由来生物
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
品質水準
製品種目
BioReagent
フォーム
aqueous solution
分子量
A subunit 400 kDa
B1 subunit 210 kDa
B2 subunit 200 kDa
包装
pkg of 1 mg
濃度
1-2 mg/mL in Tris-buffered saline
テクニック
cell culture | mammalian: suitable
表面被覆率
1‑2 μg/cm2
不純物
Microbial Contamination, passes test
NCBIアクセッション番号
結合特異性
Peptide Source: Collagen
輸送温度
dry ice
保管温度
−20°C
遺伝子情報
mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782)
類似した製品をお探しですか？ 訪問 製品比較ガイド
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当該品目 | L6274 | C0543 | 11243217001 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane) | biological source human placenta | biological source mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane) | biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) |
| concentration 1-2 mg/mL in Tris-buffered saline | concentration 0.5 mg/mL in Tris-buffered saline | concentration - | concentration - |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 100 |
| mol wt A subunit 400 kDa, B2 subunit 200 kDa, B1 subunit 210 kDa | mol wt - | mol wt - | mol wt 900 kDa |
| form aqueous solution | form liquid | form lyophilized powder | form solution |
詳細
アプリケーション
ラミニンは、上皮、内皮、神経、筋、および肝細胞を含む、多くの細胞タイプの増殖および分化をサポートします。 細胞培養足場として、1～2 μg/cm2で使用することをお勧めします。 最適な濃度は、アプリケーションや研究目的だけでなく、細胞の種類によっても異なります。
生物化学的／生理学的作用
ラミニンタンパク質は、動物組織における構造的足場に不可欠の成分です。 ラミニンは、エンタクチンやパールカンを介してタイプIVコラーゲンと会合し、インテグリン受容体、ジストログリカン糖タンパク質複合体、およびLutheran血液型糖タンパク質を通じて細胞膜に結合します。 ラミニンは、コラーゲンとの結合、細胞接着、ヘパリンとの結合、および神経突起伸長のための活性ドメインを持っています。
調製ノート
本製品は、50 mM Tris-HCl、150 mM NaClの1～2 mg/mL溶液として提供されます。 0.2 μmフィルターでろ過されています。ゲルの形成を避けるために、使用前に2～8℃でゆっくりと溶液を溶解してください。 コーティングに使用する場合は、緩衝塩溶液で希釈して、最小量で培養表面をコートし、37℃で2時間インキュベートしてください。 細胞をプレーティングする前に、PBSで3回洗浄してください。ラミニンコーティングは、2～8℃で1か月保存できます。
その他情報
ラミニンは細胞外マトリックスに存在する、マルチドメイン三量体糖タンパク質であり、接着、増殖、および分化をサポートする、主な非コラーゲン性の基底膜成分です。 ラミニンは、A、B1およびB2鎖の両方からなり、それらは多くのジスルフィド結合によって結合しています。本ラミニン製品は、マウスのエンジェルブレス−ホーム−スワーン腫瘍から精製されています。
免責事項
本製品の使用溶液のアリコートを-20°Cで保存することをお勧めします。
関連製品
製品番号
詳細
価格
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
L2020-BULK:
L2020-1MG-PW:
L2020-VAR:
L2020-1MG:
Axonal pathfinding: Extracellular matrix role
Letourneau, P
Encyclopedia of Neuroscience, 1, 1139-1145 (2009)
Mitogenic activity of laminin on human melanoma and melanocytes: different signal requirements and role of beta 1 integrins.
Mortarini R, et al.
Cancer Research, 55(20), 4702-4710 (1995)
The laminin family
Monique Aumailley
Journal of Separation Science, 7(1), 48-55 (2013)
Agnese Loda et al.
Nature communications, 8(1), 690-690 (2017-09-28)
Xist is indispensable for X chromosome inactivation. However, how Xist RNA directs chromosome-wide silencing and why some regions are more efficiently silenced than others remains unknown. Here, we explore the function of Xist by inducing ectopic Xist expression from multiple
Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor.
Thepparit C and Smith DR.
Journal of Virology, 78(22), 12647-12656 (2004)
資料
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
プロトコル
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
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How long can stocks be frozen at -20C for?
1 回答-
役に立ちましたか？
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How will the product L2020 shipped? Will it be shipped in room temperature, on ice, or on dry ice?
1 回答-
The product L2020 will be shipped using dry ice. Upon receipt, it should be stored at -20 degrees Celsius.
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How is this laminin concentration determined? (by which method?) I tried to measure the concentration of this product by ELISA, and the results always came out half of what is written on the vial.. what is the protein purity?
1 回答-
Laminin concentration is 1.0 - 2.0 mg protein/ml. The protein content is determined by Lowry method. Please refer the attached document for more details. https://www.sigmaaldrich.com/specification-sheets/131/942/L2020-1MG_________SIGMA____.pdf
役に立ちましたか？
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What type of laminin is Product L2020?
1 回答-
In the nomenclature system by Burgeson, this is Laminin 1. The laminins were numbered with Arabic numerals in the order discovered. The previous A, B1, and B2 chains, and their isoforms are listed by the Greek letters, for alpha, beta, and gamma, respectively, followed by an Arabic numeral to identify the isoforms. For example, the first laminin identified from the Engelbreth-Holm-Swarm tumor (EHS) was designated laminin-1 with the chain composition α-1/β-1/γ-1. The genes for these 3 chains are LAMA1, LAMB1, and LAMC1. Reference: Burgeson, R.E., et al., A new nomenclature for the laminins. Matrix Biol., 14, 209-211 (1994).
役に立ちましたか？
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What is the purity of Laminin, Product L2020?
1 回答-
We do not determine a purity of this product. An SDS PAGE gel is run and the pattern (3 bands) is comparable to past lots.
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What is the protein concentration of the solution of Product L2020, Laminin?
1 回答-
The protein specification for L2020 is 1.0 to 2.0 mg/mL. The exact value can be found on the lot-specific Certificate of Analysis, which can be downloaded from our website.
役に立ちましたか？
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Can I coat Laminin, Product L2020, on top of poly-L-lysine for better adherence?
1 回答-
When coating coverslips or slides for use with neural stem cells (neurospheres), the following procedure is recommended. Precoat the coverslips with poly-L-lysine (Product No. P5899) at 100 μg/mL. This is incubated for 30 minutes or more at room temperature. The coverslips are then washed with sterile water and allowed to dry. The poly-lysine coated coverslips can be stored at room temperature until ready to use (up to ~1 month). Coverslips are then coated with 30 - 50 μL of warmed laminin, which is at a concentration of 50 μg/mL. Incubate at 37°C for 30 minutes. Remove the laminin, and wash with DME (50 μL) twice. Cells are then added to the coverslips.
役に立ちましたか？
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How can I store solutions of Laminin, Product L2020?
1 回答-
The product has a recommended storage temperature of -20°C. Please note, the product needs to be thawed slowly at 2-8°C. If the product has been warmed up at room temperature and it gels, it cannot be reactivated for use. We recommend storage at 2-8°C for up to one week. For longer storage, the product needs to be frozen at -20°C in single-use aliquots.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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