CRISPR Cas9 Proteins
A Cas9 protein and synthetic guide RNA form a complex—a ribonucleoprotein (RNP)— a key to successful gene editing. Direct introduction of an RNP, rather than expression from a plasmid, offers many benefits: a functional nuclease is immediately available in the cell, then the complex is quickly cleared, making the editing activity short-lived. This reduces off-target effects and leaves no genomic footprint from DNA integration into the genome.
Figure 1.CRISPR Cas9 Base Editing
We are proud to offer a complete cytosine base editing solution for accurate and reliable gene editing. The PURedit® Cas9 Precision and Flexible Base Editor Protein Kits come with key reagents to ensure success in your experiments. PURedit® Cas9 Cytosine Base Editors allow you to avoid double-stranded breaks, minimizing off-target mutagenesis and allowing for editing in sensitive cell lines. Pair our protein kits with our synthetic guide RNAs to conveniently design and execute your genome edits. Our CRISPR design tools base editor module enables guide RNA design for both substitution edits and stop codon creation.
Choose between our two proteins to optimize your C to T substitutions.
Figure 2.Editing windows for PURedit® Cas9 Cytosine Base Editor Kits. This heat map depicts the relative likelihood of edits at different positions within the target window, with color intensity representing editing efficiency.
| PURedit® Cas9 Cytosine Base Editor Kit | Editing Window (from 5' to end of the target site) | Target Utility | Catalog Number |
|---|---|---|---|
| Precision | nt 3-9 |
| CAS9CBEPRC |
| Flexible | nt 3-15 |
| CAS9CBEFLX |
CRISPR Cas9 Proteins
Choosing the best Cas9 protein for your specific application will ensure success in your experimental goals. Whether you need a Cas9 protein with very low off-target effects, one appropriate for gene activation/inhibition studies, or one allowing visualization of delivery, we offer options that will work for you. We have the largest selection of Cas9 proteins available, so we can meet these, and many other specifications.
Some features of our Cas9 proteins include:
- More nuclear localization signals (NLSs) for specificity
- Stringent manufacturing and purification standards
- High editing activity and specificity – don’t compromise!
- Reduced off-targeting with transient expression
Once you have selected your Cas9 protein, browse our RNP protocols to see exactly how these powerful tools can work for you.
- Learn more about Cas9-GFP proteins.
- Design and order your gRNAs to complete your RNP complex:
- Design your CRISPR gRNA for standard Cas9 applications and PURedit® Cas9 Cytosine Base Editors
- Order Custom gRNA for standard Cas9 applications and PURedit® Cas9 Cytosine Base Editors
- Order Guaranteed Pre-designed gRNA
Available Proteins | ||||||
|---|---|---|---|---|---|---|
| WT Cas9 | Cas9-GFP | Cas9 Plus | Cas9 D10A (Nickase) | Cas9 D10A H840A (Dead) | PURedit® Cas9 | |
| Description | Wild-type SpCas9 protein with high gene editing activity | Wild-type SpCas9 protein fused to GFP with high gene editing activity | SpCas9 protein engineered to have both high gene editing activity and on-target specificity | Cas9 with mutation to limit cleavage to a single strand, creating a nick in DNA; requires 2 target sites for modification | Cas9 with mutations to inhibit nuclease activity but allow binding to DNA | SpCas9 protein engineered to have both highest gene editing activity and on-target specificity |
| Recommended Usage | Go-to for early evaluation when gRNA targets are screened for efficacy | Beneficial for optimizing experimental conditions Evaluate new delivery mechanisms | Ideal for gene editing experiments that require low off-target effects without compromising on-target activity | Most helpful for homology-directed repair (HDR) applications | Gene modification experiments that do not require cutting the DNA – Inhibition or Activation of gene target | Preclinical research where high efficiency and high quality are required |
| Features | 1× NLS Lyophilized | 3× NLS Enhanced GFP Lyophilized | Activity & Specificity Enhancing mutations 1× NLS Lyophilized | D10A mutation 1× NLS Lyophilized | D10A & H840A mutations 1× NLS 3× FLAG™-Biotin Tag Lyophilized | Activity & Specificity Enhancing mutations 1× NLS Lyophilized |
| Gene Editing Activity | +++ | +++ | +++ | ++ | N/A | ++++ |
| On-Target Specificity | ++ | ++ | ++++ | +++ | N/A | ++++ |
| Price | + | ++ | ++ | + | + | +++ |
| Quality Standard | ISO 9001 | ISO 9001 | ISO 9001 | ISO 9001 | ISO 9001 | ISO 9001 with enhanced quality control required for preclinical & early clinical research |
| Product Number | CAS9PROT | CAS9GFPPRO | CAS9PL | CAS9D10APR | DCAS9PROT | PECAS9 |
For additional materials to help you understand, design, and perform CRISPR experiments, click to download our Gene Editing Guide and our Molecular Essentials Guide for comprehensive tips and tools.
Related Products
Response not successful: Received status code 500
続きを確認するには、ログインするか、新規登録が必要です。
アカウントをお持ちではありませんか？