Wszystkie zdjęcia(2)

D4902

Sigma-Aldrich

Dexamethasone

powder, BioReagent, suitable for cell culture, ≥97%

Synonim(y):
9α-Fluoro-16α-methylprednisolone, 9α-Fluoro-16α-methyl-11β,17α,21-trihydroxy-1,4-pregnadiene-3,20-dione, Prednisolone F, (11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione
Empirical Formula (Hill Notation):
C22H29FO5
Numer CAS:
Masa cząsteczkowa:
392.46
Beilstein:
2066651
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.75

Poziom jakości

200

pochodzenie biologiczne

synthetic

linia produktu

BioReagent

próba

≥97%

forma

powder

siła działania

4-500 ng/mL

technique(s)

cell culture | mammalian: suitable

mp

262-264 °C (lit.)

rozpuszczalność

methanol: 25 mg/mL

transport

ambient

temp. przechowywania

2-8°C

SMILES string

C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@]4(C)[C@@]3(F)[C@@H](O)C[C@]2(C)[C@@]1(O)C(=O)CO

InChI

1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1

InChI key

UREBDLICKHMUKA-CXSFZGCWSA-N

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Zastosowanie

Dexamethasone is used to study apoptosis, cell signaling pathways, cell differentiation and gene expression.

Opakowanie

25, 100, 500 mg in glass bottle
1 g in glass bottle
Bottomless glass bottle. Contents are inside inserted fused cone.

Działania biochem./fizjol.

Glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth, and differentiation. Inhibits the induction of nitric oxide synthase.
It is poorly metabolized in the body and has great affinity and bioavailability.

Postać fizyczna

powder 2-8 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw

Rekonstytucja

To prepare 20 μg/ml stock solution, add 1ml absolute ethanol per mg product; gently swirl to dissolve. Add 49 ml sterile medium per ml of ethanol added, while mixing, to acheive final concentration of 20 μg/ml.

piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Repr. 1B

Kod klasy składowania

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certyfikat analizy

Świadectwo pochodzenia

  1. What is Product D4902, Dexamethasone, soluble in?

    Dexamethasone is practically insoluble in water. It is soluble in ethanol, methanol, acetone, dioxane and slightly soluble in chloroform. 

  2. How should I reconstitute Product D4902, Dexamethasone, for tissue culture use?

    The tissue culture grade product is dissolved in ethanol at a concentration of 1 mg/ml forming a clear, colorless solution.  It is also possible to prepare a stock solution of dexamethasone in DMSO at 1.5 mM. 

  3. Do you have references for the use of Product D4902, Dexamethasone?

    References can be found on the product detail page:Migliorati, G., et al., Dexamethasone and interleukins modulate apoptosis of murine thymocytes and peripheral T-lymphocytes. Pharmacol Res., 30(1), 43-52 (1994).Bansal, N., et al., Apoptosis: mode of cell death induced in T cell leukemia lines by dexamethasone and other agents. FASEB J. 5(2), 211-6 (1991).Shiao, R.T., et al., Dexamethasone and suramin inhibit cell proliferation and interleukin-6-mediated immunoglobulin secretion in human lymphoid and multiple myeloma cell lines. Leuk. Lymphoma, 17(5-6), 485-494 (1995).Migliorati, G., et al, Dexamethasone induces apoptosis in mouse natural killer cells and cytotoxic T lymphocytes. Immunology, 81(1), 21-26 (1994).

  4. How long is Product D4902, Dexamethasone, stable in solution?

    Dexamethasone solutions are stable for at least 30 days when stored at 4 °C and protected from light. The solution may be aliquoted and stored at -20 °C. However, insufficient information is available to assess the shelf-life of the frozen solution.

  5. What cell lines has Product D4902, Dexamethasone, been tested on?

    The cell lines on which this product is tested can vary from lot to lot. Some typically used cell lines are 3T3 fibroblasts, BHK, L929 and CHO.

  6. What is the shelf life of Product D4902, Dexamethasone?

    The product has a 5 year shelf life.

  7. Product D4902, Dexamethasone is not water soluble. Do you have a water soluble Dexamethasone?

    Product D2915 is water soluble.

  8. Can I use Product D4902, Dexamethasone, to inject into mice?

    We do not perform animal studies with our products, but it can be used in cell culture (D2915 - cell culture grade version, or USP grade product D9184).

  9. Are Products D4902 and D2915, sterile?

    No. This product is not sterile, but cell culture testing did not find the product to be toxic to cells.

  10. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  11. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  12. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  13. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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