Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growth.

Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growth.

Nanoscale research letters (2014-11-12)

Pierre-Yves Collart-Dutilleul, Ivan Panayotov, Emilie Secret, Frédérique Cunin, Csilla Gergely, Frédéric Cuisinier, Marta Martin

PMID25386101

ABSTRAKT

The way cells explore their surrounding extracellular matrix (ECM) during development and migration is mediated by lamellipodia at their leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are the primary area within the cell of actin microfilaments (filopodia) formation. In this work, we report on the use of porous silicon (pSi) scaffolds to mimic the ECM of mesenchymal stem cells from the dental pulp (DPSC) and breast cancer (MCF-7) cells. Our atomic force microscopy (AFM), fluorescence microscopy, and scanning electron microscopy (SEM) results show that pSi promoted the appearance of lateral filopodia protruding from the DPSC cell body and not only in the lamellipodia area. The formation of elongated lateral actin filaments suggests that pores provided the necessary anchorage points for protrusion growth. Although MCF-7 cells displayed a lower presence of organized actin network on both pSi and nonporous silicon, pSi stimulated the formation of extended cell protrusions.

MATERIAŁY

Numer produktu

Marka

Opis produktu

606758

Sigma-Aldrich

Formaldehyde-¹²C solution, 20% in H₂O, 99.9 atom % ¹²C

608203

Sigma-Aldrich

D-Glucose-¹²C₆, ¹⁶O₆, 99.9 atom % ¹⁶O, 99.9 atom % ¹²C

47608

Sigma-Aldrich

Formaldehyde solution, Molecular Biology, BioReagent, ≥36.0% in H₂O (T)

15512

Sigma-Aldrich

Formaldehyde solution, meets analytical specification of USP, ≥34.5 wt. %

252549

Sigma-Aldrich

Formaldehyde solution, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

340855

Sigma-Aldrich

Glutaraldehyde solution, 50 wt. % in H₂O

G6403

Sigma-Aldrich

Glutaraldehyde solution, 50% in H₂O, suitable for photographic applications

G6257

Sigma-Aldrich

Glutaraldehyde solution, Grade II, 25% in H₂O

G5882

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 25% in H₂O, specially purified for use as an electron microscopy fixative

G7526

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 8% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

G7776

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 70% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

47083-U

Supelco

Formaldehyde solution, stabilized with methanol, ~37 wt. % in H₂O, certified reference material

G7651

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 50% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

F8775

Sigma-Aldrich

Formaldehyde solution, Molecular Biology, 36.5-38% in H₂O

G5146

Sigma-Aldrich

D-(+)-Glucose, Hybri-Max^™, powder, BioReagent, suitable for hybridoma

G8270

Sigma-Aldrich

D-(+)-Glucose, ≥99.5% (GC)

G6152

Sigma-Aldrich

D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)

G5767

Sigma-Aldrich

D-(+)-Glucose, ACS reagent

G7021

Sigma-Aldrich

D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

49139

Sigma-Aldrich

D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)

49629

Sigma-Aldrich

Glutaraldehyde solution, technical, ~50% in H₂O (5.6 M)

W512303

Sigma-Aldrich

Glutaric dialdehyde solution, 50 wt. % in H₂O, FCC

49152

Sigma-Aldrich

D-(+)-Glucose, tested according to Ph. Eur.

93067

Sigma-Aldrich

Activated Charcoal Norit^®, Norit^® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed

47673

Sigma-Aldrich

Formaldehyde solution, tested according to Ph. Eur.

G7528

Sigma-Aldrich

D-(+)-Glucose, ≥99.5% (GC), BioXtra

22874

Sigma-Aldrich

Activated Charcoal Norit^®, Norit^® PK 1-3, from peat, steam activated, granular

47249

Supelco

D-(+)-Glucose, analytical standard

702102

Sigma-Aldrich

Carbon, mesoporous

699640

Sigma-Aldrich

Carbon, mesoporous, less than 100 ppm Al, Ti, Fe, Ni, Cu, and Zn combined