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MilliporeSigma

ACR5000NT

Ni-NTA Reagent Kit

suitable for protein purification

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NA.56

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product line

Amicon®

technique(s)

protein purification: suitable (His-tagged proteins)

shipped in

wet ice

General description

For use with the Amicon Pro Affinity Conc: 3 ml HisBind Ni-NTA resin, 25ml Bind Buffer (4X), 25 ml Wash buffer (4X), 5ml elution buffer (4X)
Ni-NTA Reagent Kit for His-tagged protein purification.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - Skin Corr. 1C

Storage Class

3 - Flammable liquids

flash_point_f

95.9 °F

flash_point_c

35.5 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

Related Content

Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

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