E0632
Enterokinase from porcine intestine
≥0.5 units/mg solid
Synonym(s):
Enteropeptidase
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| Size/SKU | Availability | Price |
|---|---|---|
1500 units | Available to ship TODAYfromMILWAUKEE | $458.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
≥0.5 units/mg solid
$458.00
Available to ship TODAYDetails
form
salt-free, lyophilized powder
Quality Segment
specific activity
≥0.5 units/mg solid
mol wt
150 kDa
storage temp.
−20°C
Application
The enzyme from Sigma has been used for the activation of trypsinogen in order to measure the activity of trypsin in hog pancreas. The study showed that antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure of weanling pigs.
Enterokinase from porcine intestine has been used in a study to report a new experimental model of the anomalous pancreatico-biliary junction. Enterokinase from porcine intestine has also been used in a study to investigate the insulinotropic region of the gastric inhibitory polypeptide.
Biochem/physiol Actions
Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.
Other Notes
One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| description ≥0.5 units/mg solid | description lyophilized powder, ≥100 units/mg protein | description powder | description BRP, European Pharmacopoeia (EP) Reference Standard |
| specific activity ≥0.5 units/mg solid | specific activity ≥100 units/mg protein | specific activity - | specific activity - |
| form salt-free, lyophilized powder | form lyophilized powder | form powder | form - |
| mol wt 150 kDa | mol wt 150 kDa | mol wt 42.3 kDa (+/- 6.4 kDa) | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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