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F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

ANTI-FLAG® M2 antibody, Mouse monoclonal

Synonym(s):

Monoclonal ANTI-FLAG® M2, flag antibody, Anti-ddddk, Anti-dykddddk

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50 μG

$152.00

200 μG

$557.00

1 MG

$1,210.00

5 MG

$1,830.00

$152.00

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

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This Item
F2426F4049M8823
ANTI-FLAG® M2 antibody, Mouse monoclonal 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2

Millipore

F2426

EZview™ Red ANTI-FLAG® M2 Affinity Gel

ANTI-FLAG® M2-FITC antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F4049

ANTI-FLAG® M2-FITC antibody, Mouse monoclonal

Anti-FLAG® M2 Magnetic Beads affinity isolated antibody

Millipore

M8823

Anti-FLAG® M2 Magnetic Beads

antibody form

affinity isolated antibody

antibody form

-

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

mouse

biological source

-

biological source

mouse

biological source

-

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

-

conjugate

unconjugated

conjugate

-

conjugate

FITC conjugate

conjugate

magnetic beads

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

form

-

form

buffered aqueous solution

form

suspension

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting[1], immunoprecipitation (IP) [2][3][4], immunohistochemisty, immunofluorescence[5] and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000502386
0000502386
0000502242
0000502242
0000472993

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Michael Seymour
G3 (Bethesda, Md.) (2016)
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Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)
Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as
ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins
Aparna A
Nucleic Acids Research, 45(7), 4189-4201 (2017)
MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBP?
Barbara Lipert
PLoS ONE (2017)
HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
Yalan Liu
Virology, 154-154 (2016)
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Affinity-Purifi ed ANTI-FLAG M2 Antibody with High Specifi city and Sensitivity
Product Information Sheet - - F1804
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3 Reviews This action will navigate to reviews.

Questions

1–4 of 4 Questions  

  1. Any protocol available for using the Anti-Flag M2 (F1804) in a ChIP-Seq assay? And, if the lysis buffer contains SDS and sodium deoxycholate at a concentration of 0.1%, will the presence of both denature or interfere with binding in the research process?

    1 answer

    1. Several papers have cited F1804 in ChIP & ChIPseq applications:
      https://www.nature.com/articles/s41467-023-40803-y.pdf
      https://www.nature.com/articles/s41467-023-41307-5.pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10693086&blobtype=pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10863984&blobtype=pdf

      Anti-FLAG® M2 Magnetic Beads are not compatible with deoxycholate and SDS. Please refer to compatibility chart on the datasheet linked below for further information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdf

      Helpful?

  2. Is this Ab suitable for ELISA?

    1 answer

    1. This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.

      Helpful?

  3. Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci

    1 answer

    1. Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
      https://www.nature.com/articles/s41467-019-12187-5.pdf

      Helpful?

  4. モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。

    1 answer

    1. An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf

      Helpful?

Reviews

Active Filters

  1. Technion-Israel Institute of Technology
    • Review 1
    • Votes 22
    5 out of 5 stars.

    Perfection

    Perfect for WB and IF, very specific. Stable for many years.

    Helpful?

  2. 2 Ratings-Only Reviews

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