Impaired macrophage function following bacterial stimulation in chronic granulomatous disease.

Impaired macrophage function following bacterial stimulation in chronic granulomatous disease.

Immunology (2009-09-11)

Farooq Z Rahman, Bu'Hussain Hayee, Ronnie Chee, Anthony W Segal, Andrew M Smith

PMID19740382

ABSTRACT

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for phagocyte anti-microbial activity and plays a major role in innate immunity. Defects in genes coding for components of the NADPH oxidase enzyme system are responsible for chronic granulomatous disease (CGD), a rare primary neutrophil immunodeficiency associated with recurrent, life-threatening bacterial and fungal infections. Microbial killing and digestion within the neutrophil phagosomal compartment are defective in these patients. NADPH oxidase activity is also crucial for optimal macrophage and dendritic cell function and has recently been implicated in both cross-presentation and T-cell priming. We present evidence of impaired macrophage function in CGD, with attenuated pro-inflammatory cytokine and increased interleukin-10 secretion following bacterial stimulation. These results highlight additional abnormalities in macrophage function associated with CGD and the importance of NADPH oxidase activity in immunity.

MATERIALS

Product Number

Brand

Product Description

F9423

Sigma-Aldrich

Fetal Bovine Serum, Australia origin, suitable for, USDA approved, sterile-filtered, suitable for cell culture, suitable for hybridoma

F0392

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Fetal Bovine Serum, USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture

F3297

SAFC

Fetal Bovine Serum, Australia origin, γ-irradiated, suitable for cell culture, suitable for hybridoma

F6765

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F1051

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F0926

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Fetal Bovine Serum, USDA approved, sterile-filtered, suitable for cell culture

12003C

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12306C

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Fetal Bovine Serum, non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

12103C

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Fetal Bovine Serum, USA origin, suitable for cell culture

12106C

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12107C

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12007C

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12006C

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Fetal Bovine Serum, Australia origin, USDA approved, Heat Inactivated, suitable for cell culture

F4135

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F2442

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