MilliporeSigma
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12306C

Sigma-Aldrich

Fetal Bovine Serum

non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonym(s):
HI FBS, HI FCS, HI Fetal Bovine Serum, HI Fetal Calf Sera, Heat Inactivated Fetal Calf Sera, FBS, FCS, sera, serum
MDL number:
NACRES:
NA.71

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Premier

quality

from USDA approved countries

composition

Hemoglobin, ≤25 mg/dL

origin

non-USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

−20°C

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Application

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is used as a supplement to cell culture media. It provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Suitability

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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