Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
12306C
Fetal Bovine Serum
non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture
Fetal Bovine Serum
Synonym(s):
HI FBS, HI FCS, HI Fetal Bovine Serum, HI Fetal Calf Sera, Heat Inactivated Fetal Calf Sera, FBS, FCS, sera, serum
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About This Item
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered
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biological source
bovine fetus
sterility
sterile-filtered
product line
FBS Premier
quality
from USDA approved countries
composition
Hemoglobin, ≤25 mg/dL
origin
non-USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
storage temp.
−20°C
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This Item | F0392 | 12106C | 12107C |
|---|---|---|---|
| origin non-USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
Application
FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.[1][2]
USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.
Biochem/physiol Actions
Fetal Bovine Serum (FBS) is used as a supplement to cell culture media.[3] It provides many non-defined growth promoting and survival enhancing factors to cells in culture.
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells
Asgari S, et al.
Stem Cell Reviews and Reports, 9(4), 493-504 (2013)
Shao Thing Teoh et al.
Cancer letters, 492, 21-30 (2020-08-10)
Dysregulated metabolism is a hallmark of cancer that supports tumor growth and metastasis. One understudied aspect of cancer metabolism is altered nucleotide sugar biosynthesis, which drives aberrant cell surface glycosylation known to support various aspects of cancer cell behavior including
Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture
Vosough M, et al.
Stem Cells and Development, 22(20), 2693-2705 (2013)
Gerhard Gstraunthaler
ALTEX, 20(4), 275-281 (2003-12-13)
Serum is commonly used as a supplement to cell culture media. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. The
Xiao-Min Liu et al.
Nature chemical biology, 15(9), 865-871 (2019-08-07)
RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A
Articles
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Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
Protocols
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Related Content
Explore nuestra gama de suero bovino fetal (FBS) y determine el FBS adecuado para sus requisitos y presupuesto de laboratorio. Solicite una muestra de suero.
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