Skip to Content

Love-wave sensors combined with microfluidics for fast detection of biological warfare agents.

Love-wave sensors combined with microfluidics for fast detection of biological warfare agents.

Sensors (Basel, Switzerland) (2014-07-17)

Daniel Matatagui, José Luis Fontecha, María Jesús Fernández, Isabel Gràcia, Carles Cané, José Pedro Santos, María Carmen Horrillo

ABSTRACT

The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Toluene, anhydrous, 99.8%

Sigma-Aldrich

2-Propanol, ACS reagent, ≥99.5%

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, 99%

Sigma-Aldrich

2-Propanol, anhydrous, 99.5%

Supelco

Toluene, analytical standard

Sigma-Aldrich

2-Propanol, BioUltra, Molecular Biology, ≥99.5% (GC)

Supelco

2-Propanol, analytical standard

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, ≥98%

Sigma-Aldrich

IgG from rabbit serum, technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

Sigma-Aldrich

2-Propanol, BioReagent, ≥99.5%, Molecular Biology

Sigma-Aldrich

2-Propanol, 99.5%, HPLC grade

Sigma-Aldrich

2-Propanol, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%, poly coated bottles

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, packaged for use in deposition systems, ≥98%

Supelco

Sulfuric acid concentrate, 0.1 M H₂SO₄ in water (0.2N), eluent concentrate for IC

Sigma-Aldrich

2-Propanol, JIS special grade, ≥99.5%

Sigma-Aldrich

Toluene, JIS special grade, ≥99.5%

Sigma-Aldrich

Toluene, JIS 1000, suitable for residue analysis, ≥99.8%

Sigma-Aldrich

2-Propanol, SAJ first grade, ≥99.0%

Sigma-Aldrich

2-Propanol, suitable for HPLC

Supelco

2-Propanol, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Toluene, SAJ first grade, ≥99.0%

Sigma-Aldrich

Toluene, JIS 300, suitable for residue analysis, ≥99.8%

Supelco

Toluene, HPLC grade, 99.8%

Sigma-Aldrich

2-Propanol, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, ≥98.0%

Supelco

Toluene, Pharmaceutical Secondary Standard; Certified Reference Material

USP

2-Propanol, United States Pharmacopeia (USP) Reference Standard

Sigma-Aldrich

2-Propanol, Laboratory Reagent, ≥99.5%

Sigma-Aldrich

Toluene, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Toluene, ACS reagent, ≥99.5%