Dereplication and chemotaxonomical studies of marine algae of the Ochrophyta and Rhodophyta phyla.

Dereplication and chemotaxonomical studies of marine algae of the Ochrophyta and Rhodophyta phyla.

Marine drugs (2015-05-06)

Robert Brkljača, Emrehan Semih Gӧker, Sylvia Urban

PMID25942092

ABSTRACT

Dereplication and chemotaxonomic studies of six marine algae of the Ochrophyta and one of the Rhodophyta phyla resulted in the detection of 22 separate compounds. All 16 secondary metabolites, including four new compounds (16-19), could be rapidly dereplicated using HPLC-NMR and HPLC-MS methodologies in conjunction with the MarinLit database. This study highlights the advantages of using NMR data (acquired via HPLC-NMR) for database searching and for the overall dereplication of natural products.

MATERIALS

Product Number

Brand

Product Description

574724

Sigma-Aldrich

Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid

685461

Sigma-Aldrich

Acetonitrile solution, contains 10.0% acetone, 0.05% formic acid, 40.0% 2-propanol

679186

Sigma-Aldrich

Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 5 % (v/v) water, 0.1 % (v/v) formic acid, suitable for HPLC

574732

Sigma-Aldrich

Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC

W1503

Sigma-Aldrich

Water, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture

95284

Sigma-Aldrich

Water, Molecular Biology, sterile filtered

576956

Sigma-Aldrich

Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC

W4502

Sigma-Aldrich

Water, Nuclease-Free Water, for Molecular Biology

W3513

Sigma-Aldrich

Water, BioPerformance Certified

2107

Sigma-Aldrich

E-Toxate^™ Water, endotoxin, free

W3500

Sigma-Aldrich

Water, sterile-filtered, BioReagent, suitable for cell culture

95289

Sigma-Aldrich

Water, for cell biology, sterile ultrafiltered

W1754

Sigma-Aldrich

Water, PCR Reagent

329886

Sigma-Aldrich

Water-¹⁶O, ≥99.94 atom % ¹⁶O

723851

Supelco

Dichloromethane solution, contains 10 % (v/v) methanol

437557

Sigma-Aldrich

Acetonitrile, ≥99.5%, ACS reagent

439223

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

271004

Sigma-Aldrich

Acetonitrile, anhydrous, 99.8%

270997

Sigma-Aldrich

Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

01-0620

Sigma-Aldrich

Acetonitrile, ≥99.8%, suitable for residue analysis, JIS 300

613436

Sigma-Aldrich

Deuterium oxide, 60 atom % D

01-0622

Sigma-Aldrich

Acetonitrile, ≥99.8%, suitable for residue analysis, JIS 1000

613428

Sigma-Aldrich

Deuterium oxide, 70 atom % D

07-2180

Sigma-Aldrich

Dichloromethane, JIS special grade, ≥99.0%

07-2190

Sigma-Aldrich

Dichloromethane, SAJ first grade, ≥99.0%

293040

Sigma-Aldrich

Deuterium oxide, 99.9 atom % D, contains 0.75 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

450510

Sigma-Aldrich

Deuterium oxide, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

01-0590

Sigma-Aldrich

Acetonitrile, JIS special grade, ≥99.5%

675415

Sigma-Aldrich

Acetonitrile, HPLC Plus, ≥99.9%, poly-coated bottles

01-0600

Sigma-Aldrich

Acetonitrile, SAJ first grade, ≥99.0%