Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Chemical & pharmaceutical bulletin (2015-12-04)

Yoon Kyung Kwon, Soo Jung Choi, Cho Rong Kim, Jae Kyeom Kim, Hye Kyung Kim, Jong Hun Choi, Sang-Wook Song, Chang-Ju Kim, Gwi Gun Park, Cheung-Seog Park, Dong-Hoon Shin

PMID26633030

ABSTRACT

The aim of this study was to search for a novel choline acetyltransferase (ChAT) activator from plants traditionally grown in Korea. An ethanol extract from Chaenomeles sinensis Koehne showed the highest ChAT-activating effect in vitro in an assay that used human neuroblastoma cells and [(14)C]acetyl-CoA. The active compound was speculated to be stearic acid methyl ester (SAME). In an in vivo experiment, C. sinensis extract and SAME improved trimethyltin (TMT)-induced deficits in learning and memory in mice as assessed by a Y-maze behavioral test and a passive avoidance test. The C. sinensis extract might attenuate the TMT-induced brain disorder. This study suggests that SAME from C. sinensis might be useful in the treatment of Alzheimer's disease.

MATERIALS

Product Number

Brand

Product Description

S0817

Sigma-Aldrich

Sodium chloride solution, 0.85%

59222C

SAFC

Sodium chloride solution, 5 M

204439

Sigma-Aldrich

Sodium chloride, 99.999% trace metals basis

378860

Sigma-Aldrich

Sodium chloride, random crystals, 99.9% trace metals basis

451940

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

S5150

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Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

S6546

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Sodium chloride solution, 5 M

S3014

Sigma-Aldrich

Sodium chloride, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S1679

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Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

S6150

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Sodium chloride, tablet

S8776

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Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

S7653

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Sodium chloride, BioXtra, ≥99.5% (AT)

S5886

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Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

2858

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Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)

71376

Sigma-Aldrich

Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

71386

Sigma-Aldrich

Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H₂O

S6191

Sigma-Aldrich

Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

48075

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

E2385

Supelco

Ethanol standards 10% (v/v), 10 % (v/v) in H₂O, analytical standard

28-2270

Sigma-Aldrich

Sodium chloride, JIS special grade, ≥99.5%

28-2280

Sigma-Aldrich

Sodium chloride, SAJ first grade, ≥99.0%

09-0790

Sigma-Aldrich

Ethanol, JIS special grade, 94.8-95.8%

28-2320

Sigma-Aldrich

Sodium chloride solution, 0.1 M

28-2300

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Sodium chloride solution, 1 M

09-0800

Sigma-Aldrich

Ethanol, 94.8-95.8%

E7148

Sigma-Aldrich

Ethyl alcohol, Pure 190 proof, for molecular biology

09-0832

Sigma-Aldrich

Ethanol, JIS 1000, ≥99.5%, suitable for residue analysis

09-0850

Sigma-Aldrich

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30-3302

Sigma-Aldrich

Toluene, JIS 300, suitable for residue analysis, ≥99.8%