16-282

Anti-Phosphotyrosine Antibody, clone 4G10^®, Magnetic Bead Conjugate

clone 4G10®, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4G10®, monoclonal
• 物種活性（以同源性預測）: all
• 技術: immunoprecipitation (IP): suitable
• 同型: IgG2bκ
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pTyr)
• 基因資訊: human ... PID1(55022)

描述

一般說明

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

免疫原

Phosphotyramine-KLH

應用

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

生化／生理作用

This antibody recognizes tyrosine-phosphorylated proteins from all species

外觀

Protein G-Sepharose Chromatography

20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.

Format: Purified

準備報告

Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE

分析報告

100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

法律資訊

4G10 is a registered trademark of Upstate Group, Inc.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2