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$2,680.00
$2,680.00
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Product Name
SNAP id® 2.0 Protein Detection System-Mini (7.5 x 8.4 cm), Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane..
manufacturer/tradename
SNAP id®
technique(s)
western blot: suitable
compatibility
for use with Commercially available blocking reagents
for use with Luminata Western HRP Substrates
for use with Nitrocellulose
for use with PVDF (Immobilon membranes)
for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01)
for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01)
for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001)
for use with commercially available detection reagents
detection method
chemiluminescent
colorimetric
fluorometric
shipped in
ambient
storage temp.
room temp
1 of 4
This Item | SNAP2MIDI | SNAP2MB1 | SNAP2MB3 |
|---|---|---|---|
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| manufacturer/tradename SNAP id® | manufacturer/tradename SNAP id® | manufacturer/tradename SNAP id® | manufacturer/tradename SNAP id® |
| technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable |
| compatibility for use with Commercially available blocking reagents | compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents | compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents | compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents |
| detection method chemiluminescent | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric |
General description
Application
Features and Benefits
- Processing of up to four blots at a time on a vacuum base with two individually controlled sides
- Comprises of three removable blot holding frame sizes: MultiBlot, Mini, and Midi, to accommodate different blot sizes
- Disposable blot holders, sized for MultiBlot, Mini, and Midi frames
- Blot spacer required with first-generation SNAP id system is now integrated into the new blot holder
- Extended and off-line blot processing options: frames have lids and can be removed from the base for extended incubation (one hour to overnight), incubation in a shaker, or incubation at 4 °C
- Stackable frames so multiple blots can be processed at the same time;30-minute immunodetection with uniform signal across the blot
- Greater than 80% antibody recovery using the SNAP id 2.0 Antibody Collection Trays
- Compatible with nitrocellulose and polyvinylidene fluoride (PVDF) membranes
- Works with the most blocking buffers and visualization methodologies (e.g. chemiluminescence, fluorescence, or colorimetric)
Packaging
Other Notes
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
Protocols
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
Related Content
Lo entendemos. Los Westerns pueden ser un dolor de muelas, y ciertamente hemos hecho nuestra parte. Así que, ¡comparte con nosotros tus feos borrones y recibe una sorpresa! Y aunque no podemos decirte de qué se trata -eso estropearía la sorpresa-, salvo que despertará la creatividad en el laboratorio.
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.
Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.
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