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TMKIT

mPAGE®TurboMix Bis-Tris Gel Casting Kit

Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution

Synonym(s):

Bis-Tris gel casting kit, SDS-PAGE gel casting kit, gel casting kit, protein gel casting kit

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1 KIT

$66.60

1 KIT

$129.00

$66.60

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

Key Documents

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sterility

0.2 μm filtered

form

liquid

feed ratio

29:1

storage condition

protect from light

storage temp.

2-8°C

Quality Level

200

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This Item
LUXRGTKITTMSTKMGCK
mPAGE®TurboMix Bis-Tris Gel Casting Kit Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution

Millipore

TMKIT

mPAGE®TurboMix Bis-Tris Gel Casting Kit

mPAGE® Lux Bis-Tris Reagent Kit

Millipore

LUXRGTKIT

mPAGE® Lux Bis-Tris Reagent Kit

mPAGE® TurboMix Stacking Solution 120ML Premixed stacking gel solution for casting bis-tris polyacrylamide gels

Millipore

TMSTK

mPAGE® TurboMix Stacking Solution 120ML

mPAGE® Gel Casting Device Kit for hand casting mini gels with a 0.75 mm, 1.0 mm or 1.5 mm thickness, includes plates and combs

MGCK

mPAGE® Gel Casting Device Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

storage condition

protect from light

storage condition

-

storage condition

protect from light

storage condition

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

sterility

0.2 μm filtered

sterility

-

sterility

0.2 μm filtered

sterility

-

form

liquid

form

-

form

liquid

form

-

feed ratio

29:1

feed ratio

-

feed ratio

29:1

feed ratio

-

General description

mPAGE® TurboMix Bis-Tris Gel Casting Kits deliver all the benefits of bis-tris gel chemistry. The gels operate at neutral pH which minimizes protein modification and promotes protein stability during the run. This leads to sharper bands and improved resolution. Run times are about 40% faster than standard tris-glycine gels. Cast gels can be stored at 4 °C for up to 4 weeks and are compatible with standard staining and transfer methods.
The mPAGE® TurboMix casting workflow offer many improvements over standard processes.

  • Pre-mixed acrylamide buffer solutions save time and improve reproducibility
  • Flexibility: dilute resolving solution with DI water to any gel percentage between 8% and 15%
  • Quick Cast protocol allows simultaneous polymerization of stacking and resolving gels, saving even more time

Application

Premixed solutions for casting bis-tris polyacrylamide gels.

Legal Information

MPAGE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Articles

Bis Tris Polyacrylamide Gel Electrophoresis Technology

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

Protocols

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Geles de preparación manual para PAGE y SDS-PAGE utilizando los kits de moldeo de gel bis-Tris mPAGE® TurboMix

Resumen de la química del gel de poliacrilamida e instrucciones detalladas para preparar a mano geles de poliacrilamida con los kits de moldeo del gel bis-Tris TurboMix mPAGE®

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Quick Start Guide mPAGE® TurboMix Bis-Tris Gel Casting Kit

Questions

1–3 of 3 Questions  

  1. In TMKIT mPAGE kit details 1. Flexibility: dilute resolving solution with DI water to any gel percentage between 8% and 15% Could you please confirm the stock resolving solution percentage? 2. Feed ratio 29:1 If the feed ratio indicates the ratio

    1 answer

    1. This kit may be used to prepare gels at a range of 8 - 15%. An 8% gel requires 2.4 mL Resolving Solution, a 10% requires 3 mL, a 12% requires 3.6 mL, and a 15% requires 4.5 mL. See the table below for additional details. The feed ratio refers to the amount of acrylamide monomer to crosslinker.

      Additional information may be accessed in the Product User Guide at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/613/20508849w-ms.pdf

      Helpful?

  2. Is SDS present in the resolving (TMXR-36ML) and stacking (TMXS-25ML) solutions that are apart of the kit?

    1 answer

    1. SDS is not present in the formulation.

      Helpful?

  3. What is the composition of the resolving (TMXR-36ML) and stacking (TMXS-25ML) solutions that are apart of the kit TMKIT?

    1 answer

    1. The composition is considered propreietary.

      Helpful?

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