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biological source
synthetic
Quality Level
form
lyophilized
packaging
pkg of 10 mg (11359053001)
pkg of 2 mg (10253286001)
pkg of 50 mg (11524488001)
manufacturer/tradename
Roche
technique(s)
affinity chromatography: suitable
protein purification: suitable
mp
233 °C (dec.) (lit.)
solubility
ethanol: 1 mg/mL
methanol: 1 mg/mL
acetic acid: soluble 300 μg/mL (6N)
pyridine: soluble
water: insoluble
storage temp.
2-8°C
SMILES string
CC(C)C[C@H](NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CC(C)C)C(C)C)C(C)C)[C@@H](O)CC(O)=O
InChI
1S/C34H63N5O9/c1-17(2)12-23(37-33(47)31(21(9)10)39-34(48)30(20(7)8)38-27(42)14-19(5)6)25(40)15-28(43)35-22(11)32(46)36-24(13-18(3)4)26(41)16-29(44)45/h17-26,30-31,40-41H,12-16H2,1-11H3,(H,35,43)(H,36,46)(H,37,47)(H,38,42)(H,39,48)(H,44,45)/t22-,23-,24-,25-,26-,30-,31-/m0/s1
InChI key
FAXGPCHRFPCXOO-LXTPJMTPSA-N
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General description
Pepstatin is a low molecular weight, highly specific inhibitor of acid proteases (in particular aspartate proteases).
Specificity
Application
Biochem/physiol Actions
Quality
Preparation Note
Working solution: Recommended solvent is methanol (1 mg/ml), ethanol, DMSO or glacial acetic acid.
Pepstatin is insoluble in pure water, but it is possible to produce an aqueous solutions with methanol (1.5–2.0 mg/ml), ethanol (1 mg/ml if allowed to sit overnight) or DMSO. Pepstatin is also soluble in acetic acid (6 N, 300 μg/ml) and pyridine. Solubility in NaCl solution is not known. Sodium or magnesium salts of pepstatin are water-soluble.
Stock solution: 1.4 mg pepstatin/mI (2 mM), in methanol
Storage conditions (working solution): -15 to -25 °C
Stock solutions at 1 mg/ml are stable at least a week at 4 °C. A 1 mM solution in methanol or DMSO is stable for months at -20 °C. If solutions become darker yellow, the reagent is hydrolyzing.
A working concentration of 1 μM is stable for at least one day at room temperature.
Reconstitution
Other Notes
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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