ROAHA

Anti-HA (12CA5)

from mouse IgG_2bκ

• Synonym(s): antibody
• UNSPSC Code: 12352203
• NACRES: NA.54
• Conjugate: unconjugated
• Clone: 12CA5, monoclonal
• Application: —
• Citations: 13

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 12CA5, monoclonal
• assay: >90% (HPLC and SDS-PAGE)
• form: frozen liquid (11666606001), lyophilized (11583816001)
• packaging: pkg of 200 μg (11583816001), pkg of 5 mg (11666606001 [1 ml])
• manufacturer/tradename: Roche
• concentration: 5 mg/ml (11666606001)
• isotype: IgG2bκ
• epitope sequence: YPYDVPDYA
• storage temp.: −20°C
• Quality Level: 100

Description

Analysis Note

Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.

Application

Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:

• Dot blots
• Immunochemistry[1]
• Immunoprecipitation[2]
• Western blotting[3]

Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).

Biochem/physiol Actions

Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection.[4] The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.[5]

General description

Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease.[5] The HA gene is mapped to segment 4 of influenza B viral genome.[6]

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.

Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

• Dot blot: 0.1 to 1 μg/ml
• Immunfluorescence: 1-10 μg/ml
• Western blot: 0.1 to 1 μg/ml

Safety Information

• Storage Class: 13 - Non Combustible Solids
• flash_point_f: does not flash
• flash_point_c: does not flash