TOSRTRO
Roche
Transcriptor One-Step RT-PCR Kit
sufficient for 50 reactions, sufficient for 150 reactions, suitable for RT-qPCR, suitable for RT-PCR, hotstart
Recommended Products
usage
sufficient for 150 reactions
sufficient for 50 reactions
Quality Level
feature
hotstart
manufacturer/tradename
Roche
packaging
pkg of 150 reactions (04655885001)
pkg of 50 reactions (04655877001)
technique(s)
RT-PCR: suitable
RT-qPCR: suitable
input
purified RNA
detection method
probe-based
Related Categories
General description
The Transcriptor One-Step RT-PCR Kit offers the outstanding benefits of Transcriptor Reverse Transcriptase, as well as the advantages of a hot start one-step RT-PCR format.
The kit′s RT-PCR Enzyme Mix contains four different enzymes: Transcriptor Reverse Transcriptase ensures sensitive and robust reverse transcription with high yield; Protector RNase Inhibitor, fully active at elevated temperatures, provides maximum template protection during reverse transcription; and the Expand System – a blend consisting of Taq DNA Polymerase and a proofreading polymerase – minimizes the possibility of mutations, offering high yield and fidelity in the PCR.
The optimized RT-PCR Reaction Buffer includes high quality dNTPs and provides the overall improved performance of a hot start system. The buffer′s unique hot start component binds and sequesters primers at lower temperatures to prevent the primers from binding to nonspecific sites. Another component binds magnesium, in a temperature-dependent manner, to prevent uncontrolled DNA synthesis. This new buffer formulation is effective during reverse transcription as well as PCR, resulting in increased specificity and sensitivity.
The combination of optimized reaction buffer and enzyme mix ensures efficient transcription of difficult templates with high secondary structure and GC content without increasing reaction temperature.
Application
Featuring an innovative reaction buffer, the kit combines sensitive, high-yield reverse transcription with the improved performance of a hot start system for high-fidelity, high-yield amplification of a variety of templates, including GC-rich RNA.
The kit offers a convenient, highly sensitive one-step RT-PCR method to generate transcripts up to 6.5 kb for applications such as:
- Qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels
- Determining the presence or absence of RNA templates
- Quantifying gene expression through RNA analysis (e.g., for virus-level quantification)
- Cloning of RNA up to 6.5 kb
The one-step format is convenient, reduces reaction-to-reaction variability, and prevents contamination by minimizing hands-on steps.
- hepatitis C virus (HCV) fragments
- heavy chain CH3 region and light chain polyA region
- murine leukemia virus (MLV)
Features and Benefits
- Obtain high sensitivity and yield in a wide amplification range:
- Achieve high specificity and reduce primer-dimers
- Produce long fragments:
- Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA):
- Save time and improve results with a convenient kit:
- Protect your RNA sample:
- Increase confidence in your results:
Components
- RT-PCR Enzyme Mix containing Transcriptor Reverse Transcriptase, Protector RNase Inhibitor, and the Expand System
- RT-PCR Reaction Buffer containing hot start mediating components and dNTPs – 5x concentrated
- Water, PCR Grade
- Control RNA – HAV RNA, in vitro transcript *
- Control primer mix HAV RNA (forward and reverse primer) *
* Included only in the 50-reaction pack size.
Quality
In addition, RT-PCR is performed on a dilution series of human liver total RNA (10 ng, 1 ng, and 0.1 ng). RT-PCR with specific primers for a 2.5 kb fragment of ApoB is performed using the standard RT-PCR protocol (reverse transcription at +50°C for 30 minutes) provided in the package insert. With 1 ng of RNA a clearly visible band is obtained after agarose gel electrophoresis and ethidium bromide staining.
Other Notes
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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