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MilliporeSigma

A3151

Avidin–Peroxidase

lyophilized powder

Synonym(s):

Avidin-POD

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$152.00

2 MG

$259.00

5 MG

$527.00

$152.00

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About This Item

MDL number:
MFCD00130587
UNSPSC Code:
12352203
NACRES:
NA.46

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biological source

avidin from egg white
enzyme from horseradish

Quality Level

200

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL
PBS: soluble

storage temp.

−20°C

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This Item
A7294189725A2050
Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin, Egg White

Sigma-Aldrich

189725

Avidin, Egg White

Avidin–FITC from egg white buffered aqueous solution

Sigma-Aldrich

A2050

Avidin–FITC from egg white

biological source

avidin from egg white, enzyme from horseradish

biological source

avidin from egg white, enzyme from bovine (calf) intestine

biological source

-

biological source

-

technique(s)

direct ELISA: 1:50,000

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

technique(s)

-

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized solid

form

buffered aqueous solution

solubility

H2O: soluble 1 mg/mL, PBS: soluble

solubility

-

solubility

dilute buffer: 5 mg/mL, distilled water: 5 mg/mL

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

-

conjugate

FITC conjugate

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding[2] and OVA-IgG1[3].
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM5375
SLCD1995
SLCB6957
SLBW7331
SLBV8230

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Anton B Iliuk et al.
Current protocols in chemical biology, 7(1), 17-25 (2015-03-03)
This article describes methods for a new, non-antibody phosphorylation detection reagent, termed pIMAGO (phospho-imaging). This novel reagent takes advantage not only of the unique properties of the soluble nanoparticles, but also of the multiple functionalities of the molecule, allowing for
D G Alleva et al.
Diabetes, 49(7), 1106-1115 (2000-07-26)
Cytokines derived from macrophages (Mø) play a critical role in the development of type 1 diabetes in the nonobese diabetic (NOD) mouse. Based on earlier findings from lupus-prone strains of inherent cytokine defects in Mø , NOD Mø were evaluated
S de la Barrera et al.
Clinical and experimental immunology, 138(1), 128-138 (2004-09-18)
Activation of T cells requires both TCR-specific ligation and costimulation through accessory molecules during T cell priming. IFNgamma is a key cytokine responsible for macrophage activation during Mycobacterium tuberculosis (Mtb) infection while IL-10 is associated with suppression of cell mediated
Peter Patrikios et al.
Brain : a journal of neurology, 129(Pt 12), 3165-3172 (2006-08-22)
Although spontaneous remyelination does occur in multiple sclerosis lesions, its extent within the global population with this disease is presently unknown. We have systematically analysed the incidence and distribution of completely remyelinated lesions (so-called shadow plaques) or partially remyelinated lesions
P Birner et al.
The American journal of pathology, 158(6), 1991-1996 (2001-06-08)
Monoclonal antibody MIB-1 is a reliable tool for determining proliferating cells in human tissues, but does not react with the homologous mouse antigen and is therefore useless in experimental pathology using mice as model systems. Standard method for assessment of
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