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  • B7906
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B7906

Sigma-Aldrich

1-Butanol

for molecular biology, ≥99%

Synonym(s):
n-Butanol, Butyl alcohol
Linear Formula:
CH3(CH2)3OH
CAS Number:
Molecular Weight:
74.12
Beilstein:
969148
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

vapor density

2.55 (vs air)

vapor pressure

5 (4 mmHg) at 20 °C hPa

Assay

≥99%

form

liquid

autoignition temp.

649 °F

expl. lim.

11.2 %

color

colorless

refractive index

n20/D 1.399 (lit.)

pH

7 (20 °C, 70 g/L)

bp

116-118 °C (lit.)

mp

−90 °C (lit.)

density

0.81 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

CCCCO

InChI

1S/C4H10O/c1-2-3-4-5/h5H,2-4H2,1H3

InChI key

LRHPLDYGYMQRHN-UHFFFAOYSA-N

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1-Butanol for molecular biology, ≥99%

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1-Butanol

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1-Butanol for liquid chromatography LiChrosolv®

Supelco

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1-Butanol

1-Butanol 99.9%

Sigma-Aldrich

537993

1-Butanol

grade

for molecular biology

grade

ACS reagent

grade

-

grade

-

vapor density

2.55 (vs air)

vapor density

2.55 (vs air)

vapor density

-

vapor density

2.55 (vs air)

vapor pressure

5 (4 mmHg) at 20 °C hPa

vapor pressure

5.5 mmHg

vapor pressure

6.7 hPa ( 20 °C)

vapor pressure

5.5 mmHg

assay

≥99%

assay

≥99.4%

assay

≥99.8% (GC)

assay

99.9%

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

649 °F

autoignition temp.

649 °F

autoignition temp.

340 °C

autoignition temp.

649 °F

General description

1-Butanol is a clear, colorless alcohol that can be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation. It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions, thus improving recovery by ethanol precipitation.

Application

1-Butanol has been used:
  • in the derivatization process for measuring methylated arginine levels in blood samples
  • in liquid-chromatography mass spectrometry (LC-MS) analysis of lysine modifications by deacetylase CobB
  • in the extraction of plant material from Vigna unguiculata
  • to extract and purify amplified human DNA samples before further amplification by PCR followed by sequencing

1-Butanol is suitable for use in nucleic acid purification and concentration protocols.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

95.0 °F - Pensky-Martens closed cup

Flash Point(C)

35 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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