If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
D141
Digitonin
Used as non-ionic detergent
Synonym(s):
Digitin
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$87.20
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$87.20
In StockDetails
biological source
plant seeds (Digitalis purpurea)
form
powder
optical activity
[α]20/D −54°, c = 2.8 in methanol(lit.)
mol wt
micellar avg mol wt 70,000
aggregation number
60
impurities
<6.0% water
CMC
<0.5 mM (20-25°C)
<0.5 mM (20-25°C)
mp
230-240 °C (dec.) (lit.)
solubility
H2O: ~5 % (w/v) (solubilized by heating to 95 °C - 98 °C and then cooling to room temp.)
SMILES string
C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H
InChI
1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1
InChI key
UVYVLBIGDKGWPX-KUAJCENISA-N
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Related Categories
1 of 4
This Item | D5628 | 1199002 | PHL82516 |
|---|---|---|---|
| form powder | form powder | form - | form - |
| aggregation number 60 | aggregation number 60 | aggregation number - | aggregation number - |
| mp 230-240 °C (dec.) (lit.) | mp 230-240 °C (dec.) (lit.) | mp 240 °C (dec.) (lit.) | mp 240 °C (dec.) (lit.) |
| biological source plant seeds (Digitalis purpurea) | biological source plant seeds (Digitalis purpurea) | biological source - | biological source - |
| impurities <6.0% water | impurities - | impurities - | impurities - |
| solubility H2O: ~5 % (w/v) (solubilized by heating to 95 °C - 98 °C and then cooling to room temp.) | solubility ethanol: 10 mg/mL, clear, colorless to faintly yellow | solubility - | solubility - |
General description
Moreover, Digitonin selectively permeabilizes the cholesterol-rich plasma membrane, leaving organelle membranes intact. This feature facilitates the extraction and purification of specific organelles, contributing to the study of their functions and roles in cellular processes. The detergent′s capability to permeabilize diverse cell types allows the study of intracellular components and processes by introducing molecules, antibodies, or enzymes into cells. Additionally, Digitonin finds application in immunocytochemistry experiments, enabling the labeling and detection of intracellular proteins and structures.
Application
Biochem/physiol Actions
Features and Benefits
Quality
Other Notes
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - STOT RE 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the stability of D141 and N7537 in the stock solution in DMSO?
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D141 can remain stable for up to one week when refrigerated, whether in water or DMSO. On the other hand, N7537 can be stored in DMSO (or ethanol) at -20°C for up to two weeks.
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Wie kann ich das Produkt vor den Experiment aufreinigen? haben Sie ein Protokoll dazu? danke Beste Grüße Ayoub
1 answer-
There is no specific protocol for the purification of item D141 available. The quality of this product is already detailed as being free of water-insoluble constituents. The purpose of the purification would be to remove these water-insoluble impurities.
This is usually done through a recrystallization technique. This product will dissolve at 1 g in 20 mL of water at 95 - 98°C, as detailed on the corresponding specification sheet:
https://www.sigmaaldrich.com/specification-sheet/SIGMA/D141Thus, it should be dissolved directly in water with high heat without any prior purification.
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What is the storage condition for digitonin ?
1 answer-
This product should be stored in room temperature. Please keep the container tightly closed in a dry and well-ventilated place. To view safety handling information, please view the safety data sheet for the product:
https://www.sigmaaldrich.com/sds/sigma/d141Helpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How pure is Product D141, Digitonin?
1 answer-
The purity of this product is evaluated by Thin Layer Chromatography. The actual results are lot specific and will be reported on the Certificate of Analysis of each lot.
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What is the solubility of Product D141, Digitonin?
1 answer-
The solubility of D141 is tested by heating a 5% solution in water to 95-98°C and then cooling the solution to room temperature. After cooling, the solution should remain practically clear for at least 24 hours. The product can also be dissolved at 100mg/4mL of warm ethanol.
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What is the solution stability of Product D141, Digitonin?
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We recommend making solutions fresh for use.
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