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D5652

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

Synonym(s):

DPBS

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10 L
$23.90
10 X 1 L
$40.30
50 L
$82.00

About This Item

MDL number:
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.25

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$23.90


In StockDetails


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Technical Service
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Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

pH

7.2-7.8

solubility

water: 9.6 g/L, clear

storage temp.

2-8°C

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1 of 4

This Item
D1283D8662D4031
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

500

Quality Level

400

Quality Level

400

Quality Level

200

form

powder

form

liquid

form

liquid

form

liquid

solubility

water: 9.6 g/L, clear

solubility

-

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

pH

7.2-7.8

pH

-

pH

-

pH

-

General description

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

Application

D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy[1]
  • to dissolve Angelica gigas powder for microencapsulation[2]
  • to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay[3]
  • to wash amniotic membrane for cellular analyses[4]

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

1–7 of 7 Questions  
  1. What is the formulation for this buffer? Molarity of Na2HPO4, KH2PO4, NaCl, KCl, etc?

    1 answer
    1. See the link below to review the formulations of the DPBS products. These concentrations are reported in grams per liter:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/269/385/d5652for.pdf

      Helpful?

  2. What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?

    1 answer
    1. This product contains 8.0 g/L sodium chloride.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?

    1 answer
    1. The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.

      Helpful?

  5. Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?

    1 answer
    1. Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use.  It can be autoclaved at 121°C, 15 psi for 20 minutes.

      Helpful?

  6. Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?

    1 answer
    1. Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.

      Helpful?

  7. Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?

    1 answer
    1. Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.

      Helpful?

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