Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

MDL number:




cell culture | mammalian: suitable




water: 9.6 g/L, clear

storage temp.


Looking for similar products? Visit Product Comparison Guide

Related Categories


D-PBS is used to wash cells during preparation and serial transfer.


Formulated to contain 9.6 grams of powder per liter of medium.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?

    Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.

  6. Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?

    Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use.  It can be autoclaved at 121°C, 15 psi for 20 minutes.

  7. Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?

    Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.

  8. What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?

    This product contains 8.0 g/L sodium chloride.

  9. What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?

    The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ana C Bohórquez et al.
Journal of colloid and interface science, 506, 393-402 (2017-07-28)
The rotational diffusion of polyethylene glycol coated magnetic nanoparticles in serum albumin solutions was investigated in a range spanning 0mgmL
Zhaoxia Zhang et al.
Molecular vision, 24, 434-442 (2018-07-24)
Retinal G protein-coupled receptor (RGR) mRNA is transcribed in the outer nuclear layer of human retinas; however, it is not known whether the RGR gene is expressed in the rod or cone photoreceptors. In this study, we investigate broader expression...
Barbora Bílková et al.
Veterinary immunology and immunopathology, 188, 71-77 (2017-06-16)
While haematological variation is well known in birds, variation in avian breeds (distinct morphotypes of the same species) remains unexplored. Poultry breeds, in particular, may show interesting evolutionary patterns and economically-relevant physiological differences. We performed a comparative examination of blood...
Harold Kochounian et al.
Molecular vision, 22, 213-223 (2016-03-25)
Rare mutations in the human RGR gene lead to autosomal recessive retinitis pigmentosa or dominantly inherited peripapillary choroidal atrophy. Here, we analyze a common exon-skipping isoform of the human retinal G protein-coupled receptor opsin (RGR-d) to determine differences in subcellular...
Iqbal Hyder et al.
BMC molecular and cell biology, 21(1), 9-9 (2020-03-01)
Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited proliferative capacity....

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.