MAC4L
MetaPolyzyme
lyophilized powder
Synonym(s):
Multilytic Enzyme Mix
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
lyophilized powder
Quality Level
technique(s)
DNA extraction: suitable
suitability
suitable for microbiology
application(s)
microbiology
shipped in
wet ice
storage temp.
−20°C
General description
Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.
Application
MetaPolyzyme is a mixture of 6 enzymes that is intended for isolation of total DNA for metagenomics studies. Adapted from an initial formulation devised by S. Tighe,2 MetaPolyzyme was evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG). Test data on the use of MetaPolyzyme is accessible at the 2017 ABRF MGRG Poster.
Biochem/physiol Actions
Microbes can be difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted by using lysing enzymes (such as lyticase or chitinase) to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. MetaPolyzyme is intended for use in metagenomic studies, such as study of microbes and microbiomes of extreme and unique environments. MetaPolyzyme is intended for digestion of microbes for evaluation by whole genome shotgun sequencing for metagenomics and metatranscriptomic approaches.
Components
The enzymes in MetaPolyzme are:
- Achromopeptidase
- Chitinase
- Lyticase
- Lysostaphin
- Lysozyme
- Mutanolysin
Unit Definition
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Microbiome, 4(1), 24-24 (2016-06-04)
The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium is a novel, interdisciplinary initiative comprised of experts across many fields, including genomics, data analysis, engineering, public health, and architecture. The ultimate goal of the MetaSUB Consortium
Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children.
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The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium inaugural meeting report.
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Journal of biomolecular techniques : JBT, 28(1), 31-39 (2017)
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Articles
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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