MilliporeSigma
All Photos(1)

MINI26

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Synonym(s):
Red PKH membrane label kit
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

technique(s)

flow cytometry: suitable

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

General description

This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Application

PKH26 red fluorescent cell linker mini kit has been used for:
  • labelling microglial, natural killer cells and preadipocytes
  • tracking of viable transplanted adipose-derived stem cells
  • in vitro proliferation studies of bone marrow stem cells
  • general cell membrane labeling, including for in vitro cell labeling and long term in vivo cell tracking.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description

  • Diluent C 10 mL

  • PKH26 Cell Linker in ethanol .1 mL

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14 °C - closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Imaging of peripheral benzodiazepine receptor expression as biomarkers of detrimental versus beneficial glial responses in mouse models of Alzheimer's and other CNS pathologies
Ji B, et al.
The Journal of Neuroscience, 28(47), 12255-12267 (2008)
Transplanted bone marrow stem cells relocate to infarct penumbra and co-express endogenous proliferative and immature neuronal markers in a mouse model of ischemic cerebral stroke
Zhang XM, et al.
BMC Neuroscience, 11(1), 138-138 (2010)
Adipose tissue regeneration in vivo using micronized acellular allogenic dermis as an injectable scaffold
Lee HY, et al.
Aesthetic Plastic Surgery, 38(5), 1001-1010 (2014)
Maria Giovanna Gandolfi et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-03-04)
Exosomes derived from mesenchymal stem cells are extracellular vesicles released to facilitate cell communication and function. Recently, polylactic acid (PLA), calcium silicates (CaSi), and dicalcium phosphate dihydrate (DCPD) have been used to produce bioresorbable functional mineral-doped porous scaffolds-through thermally induced
Human leukocyte antigen-G1 inhibits natural killer cytotoxicity through blocking the activating signal transduction pathway and formation of activating immunologic synapse
Yu Y, et al.
Human immunology, 69(1), 16-23 (2008)

Articles

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

A video about how you can use fluorescent cell tracking dyes in combination with flow and image cytometry to study interactions and fates of different cell types in vitro and in vivo.

Cell Tracking with Lipophilic Membrane Dyes

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service