P9155

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Synonym(s): PLL HBr
• CAS Number: 25988-63-0
• UNSPSC Code: 12352202
• eCl@ss: 32160406
• PubChem Substance ID: 24899014
• NACRES: NA.75
• MDL number: MFCD00237230

Properties

• Product Name: Poly-L-lysine hydrobromide, mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChI key: WBSCNDJQPKSPII-KKUMJFAQSA-N
• biological source: synthetic (organic)
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• mol wt: 30,000-70,000
• packaging: pkg of 5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 4 μg/cm²
• solubility: H₂O: soluble 50 mg/mL, clear, colorless
• shipped in: ambient
• storage temp.: −20°C
• Quality Level: 200

Description

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Application

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.[1]
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates.[2] It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.[3]

Biochem/physiol Actions

Poly-L-lysine is employed as a coating substance to facilitate improved cell attachment to culture surfaces.[4] Research indicates that plates coated with poly-L-lysine (PLL) exhibit an accelerated cell growth rate.[5] When mesenchymal stem cells (MSCs) are cultured on PLL-coated substrates, it helps preserve their stemness properties and prevents senescence. PLL-coated plates promote enhanced MSC growth and up-regulation of genes associated with cell adhesion, differentiation, proliferation, and signaling. Furthermore, porous PLL-coated surfaces have been shown to enhance cell proliferation and support the osteogenic differentiation of MSCs.[6]

Disclaimer

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.[1]

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)