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TP0100

Sigma-Aldrich

Total Protein Kit, Micro

test parameters

wavelength: 595 nm
total test time: 2 min
working reagent stability 18-26°C: 2 weeks
dynamic range: 50 mg/dL, 0.5 g/L
sample volume: 50 μL
temp.: 18-26 °C

Quality Level

storage temp.

2-8°C

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Suitability

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principle

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Testing Method

Colorimetric, Endpoint

Kit Components Also Available Separately

Product No.
Description
SDS

  • Protein Standard 5 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

132.8 °F

Flash Point(C)

56 °C

Certificate of Analysis

Certificate of Origin

Elizabeth Gray et al.
Journal of neuropathology and experimental neurology, 67(9), 888-899 (2008-08-22)
Matrix metalloproteinases (MMPs) degrade extracellular matrix; MMP activity, particularly of MMP-9, is elevated in the white matter in multiple sclerosis (MS) patients. The cerebral cortical extracellular matrix includes perineuronal nets (PNs) that surround parvalbumin-positive neurons (PV-positive neurons) and are important
Francisca P Martínez-Antequera et al.
Animals : an open access journal from MDPI, 11(6) (2021-07-03)
This study evaluated the use of Ulva ohnoi as an ingredient in feeds for aquaculture in three different experiments. Experiment 1 was oriented to confirm the negative effect of U. ohnoi on fish digestion. Experiment 2 assessed the effect on
Synthesis, molecular modelling and biological activity of some pyridazinone derivatives as selective human monoamine oxidase-B inhibitors.
Ozdemir, et al.
Pharmacological Reports, 72, 692-704 (2021)
Digital camera-based lipase biosensor for the determination of paraoxon
Pohanka M, et al.
Sensors and Actuators B, Chemical, 273, 610-615 (2018)
Jason G Lieberthal et al.
The Journal of rheumatology, 40(5), 674-683 (2013-04-03)
Glomerulonephritis (GN) is common in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), but tools for early detection of renal involvement are imperfect. We investigated 4 urinary proteins as markers of active renal AAV: alpha-1 acid glycoprotein (AGP), kidney injury molecule-1 (KIM-1)

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