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TP0100

Sigma-Aldrich

Total Protein Kit, Micro

test parameters

wavelength: 595 nm
total test time: 2 min
working reagent stability 18-26°C: 2 weeks
dynamic range: 50 mg/dL, 0.5 g/L
sample volume: 50 μL
temp.: 18-26 °C

Quality Level

storage temp.

2-8°C

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Suitability

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principle

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Testing Method

Colorimetric, Endpoint

Kit Components Also Available Separately

Product No.
Description
SDS

  • Protein Standard 5 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

Storage Class Code

3 - Flammable liquids

WGK Germany

WGK 3

Flash Point(F)

132.8 °F

Flash Point(C)

56 °C

Certificate of Analysis

Certificate of Origin

null
Joshua M Halman et al.
Tree physiology, 33(11), 1242-1251 (2013-12-05)
Forests of northeastern North America have been exposed to anthropogenic acidic inputs for decades, resulting in altered cation relations and disruptions to associated physiological processes in multiple tree species, including sugar maple (Acer saccharum Marsh.). In the current study, the...
Jason G Lieberthal et al.
The Journal of rheumatology, 40(5), 674-683 (2013-04-03)
Glomerulonephritis (GN) is common in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), but tools for early detection of renal involvement are imperfect. We investigated 4 urinary proteins as markers of active renal AAV: alpha-1 acid glycoprotein (AGP), kidney injury molecule-1 (KIM-1)...
Synthesis, molecular modelling and biological activity of some pyridazinone derivatives as selective human monoamine oxidase-B inhibitors.
Ozdemir, et al.
Pharmacological Reports, 72, 692-704 (2021)
Elizabeth Gray et al.
Journal of neuropathology and experimental neurology, 67(9), 888-899 (2008-08-22)
Matrix metalloproteinases (MMPs) degrade extracellular matrix; MMP activity, particularly of MMP-9, is elevated in the white matter in multiple sclerosis (MS) patients. The cerebral cortical extracellular matrix includes perineuronal nets (PNs) that surround parvalbumin-positive neurons (PV-positive neurons) and are important...

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