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HPLC Method Transfer Calculator

Calculate the saving in run time and solvent consumption when transferring a method from HPLC conditions to UHPLC conditions. Obtain guidance on change in back pressure as well as how to adapt injection volume and gradient conditions.

Reset
Reset
Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
Reset
Reset
Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Equilibration
Time(min)
17.44
3.49
4.04
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
Gradient
Conditions
Current Method
New Method
New MethodAdjust Dwell Volume
Dwell Volume(mL)
Gradient Delay(min)
0.00
0.00
Gradient
Table
TIME
% A
% B
New Time
New TimeAdjusted Flow
20
0.00
0.00
20
0.40
0.46
50
2.00
2.32
50
4.00
4.63
20
5.00
5.79
20
6.00
6.95
+ Insert Row
+ Insert Row
- Delete Last Row
- Delete Last Row


The scaled gradient method is based on estimates of column volumes and is therefore only an approximation. More precise method scaling requires actual measured values of column volumes. Column volume is a function of particle size, particle structure, and column packing.

If you have questions or comments about this tool, please fill out the form below to share your feedback.

Reset
Reset
Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
Reset
Reset
Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Equilibration
Time(min)
17.44
3.49
4.04
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
Gradient
Conditions
Current Method
New Method
New MethodAdjust Dwell Volume
Dwell Volume(mL)
Gradient Delay(min)
0.00
0.00
Gradient
Table
TIME
% A
% B
New Time
New TimeAdjusted Flow
20
0.00
0.00
20
0.40
0.46
50
2.00
2.32
50
4.00
4.63
20
5.00
5.79
20
6.00
6.95
+ Insert Row
+ Insert Row
- Delete Last Row
- Delete Last Row


The scaled gradient method is based on estimates of column volumes and is therefore only an approximation. More precise method scaling requires actual measured values of column volumes. Column volume is a function of particle size, particle structure, and column packing.

If you have questionns or comments about this tool, please click here to share your feedback.

HPLC Resources


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Analyze small molecules, such as amino acids, lipids, sugars, fatty acids and alkaloids.

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