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  • Crystal structure of a conger eel galectin (congerin II) at 1.45A resolution: implication for the accelerated evolution of a new ligand-binding site following gene duplication.

Crystal structure of a conger eel galectin (congerin II) at 1.45A resolution: implication for the accelerated evolution of a new ligand-binding site following gene duplication.

Journal of molecular biology (2002-09-11)
Tsuyoshi Shirai, Yuuka Matsui, Clara Shionyu-Mitsuyama, Takashi Yamane, Hisao Kamiya, Chihiro Ishii, Tomohisa Ogawa, Koji Muramoto
ABSTRACT

The crystal structure of congerin II, a galectin family lectin from conger eel, was determined at 1.45A resolution. The previously determined structure of its isoform, congerin I, had revealed a fold evolution via strand swap; however, the structure of congerin II described here resembles other prototype galectins. A comparison of the two congerin genes with that of several other galectins suggests acceralated evolution of both congerin genes following gene duplication. The presence of a Mes (2-[N-morpholino]ethanesulfonic acid) molecule near the carbohydrate-binding site in the crystal structure points to the possibility of an additional binding site in congerin II. The binding site consists of a group of residues that had been replaced following gene duplication suggesting that the binding site was built under selective pressure. Congerin II may be a protein specialized for biological defense with an affinity for target carbohydrates on parasites' cell surface.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MES hydrate, BioPerformance Certified, suitable for cell culture, ≥99.5%
Sigma-Aldrich
MES hydrate, ≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra
Sigma-Aldrich
MES hydrate, ≥99.5% (titration)
Sigma-Aldrich
MES hydrate, BioUltra, ≥99.5% (T)
Sigma-Aldrich
MES solution, BioUltra, for molecular biology, 0.5 M in H2O
Sigma-Aldrich
MES sodium salt, BioReagent, ≥99% (titration), crystalline
Sigma-Aldrich
MES sodium salt, BioPerformance Certified, suitable for cell culture
Sigma-Aldrich
MES, low moisture content, ≥99% (titration)
Sigma-Aldrich
MES sodium salt, ≥99% (titration)
SAFC
MES sodium salt