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INT-dehydrogenase activity test for assessing anaerobic biodegradability of organic compounds.

Ecotoxicology and environmental safety (2002-12-18)
Yang Hongwei, Jiang Zhanpeng, Shi Shaoqi, W Z Tang
ABSTRACT

This study assessed anaerobic biodegradability of organic compounds from microorganism activity. Dehydrogenase activity can be a good parameter characterizing the microorganism activity. A modified method of 2-(p-iodophenyl-3-(p-nitrophenyl)-5-pheny tetrazolium chloride-dehydrogenase activity determination was proposed in anaerobic biodegradability assessment. Cubic spline curves were adopted to link the data points. This curve was integrated twice to calculate areas. The microorganism activity index in anaerobic biodegradability assessment was calculated by standardizing the integral. According to the results of the activity index, 14 kinds of organic compounds were classified into readily, partially, and poorly biodegradable under anaerobic conditions, respectively. As a result, some conclusions for anaerobic biodegradability of organic compounds were reached, based on the activity index value.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iodonitrotetrazolium chloride, BioReagent, ≥97.0% (calc. on dry substance, NT)
Sigma-Aldrich
Iodonitrotetrazolium chloride, Used in colorimetric assays.
Sigma-Aldrich
Iodonitrotetrazolium chloride, 95%