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Simultaneous determination of nitroglycerin and its dinitrate metabolites by capillary gas chromatography with electron-capture detection.

Journal of chromatography (1992-05-20)
M Jørgensen, M P Andersen, S H Hansen
ABSTRACT

A major problem in the analysis of nitroglycerin (propanetriol trinitrate, GTN) and its metabolites [propanetriol 1,2-dinitrate (1,2-GDN) and propanetriol 1,3-dinitrate (1,3-GDN)] has been the adsorption of the nitro compounds to glass apparatus during sample preparation or injection. In this paper we present a method determining GTN, 1,2-GDN and 1,3-GDN in plasma at low nanomolar level using capillary gas chromatography with electron-capture detection. The detection limits are about 0.2 nM in plasma. Adsorption of the nitro compounds to glassware is prevented by using triethylamine, giving a simple sample preparation and accurate results. Thus, the laborious and time-consuming silanization of the glassware is avoided.

MATERIALS
Product Number
Brand
Product Description

Supelco
1,3-Dinitroglycerin solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Supelco
1,3-Dinitroglycerin solution, 100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®